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dc.contributor.author
Burgos, Juan Ignacio
dc.contributor.author
Yeves, Alejandra del Milagro
dc.contributor.author
Barrena, Jorge P.
dc.contributor.author
Portiansky, Enrique Leo
dc.contributor.author
Vila Petroff, Martin Gerarde
dc.contributor.author
Ennis, Irene Lucia
dc.date.available
2018-06-20T01:30:16Z
dc.date.issued
2017-11
dc.identifier.citation
Burgos, Juan Ignacio; Yeves, Alejandra del Milagro; Barrena, Jorge P.; Portiansky, Enrique Leo; Vila Petroff, Martin Gerarde; et al.; Nitric oxide and CaMKII: critical steps in the cardiac contractile response To IGF-1 and swim training; Elsevier; Journal of Molecular and Cellular Cardiology; 112; 11-2017; 16-26
dc.identifier.issn
0022-2828
dc.identifier.uri
http://hdl.handle.net/11336/49487
dc.description.abstract
Cardiac adaptation to endurance training includes improved contractility by a non-yet clarified mechanism. Since IGF-1 is the main mediator of the physiological response to exercise, we explored its effect on cardiac contractility and the putative involvement of nitric oxide (NO) and CaMKII in control and swim-trained mice.IGF-1 increased cardiomyocyte shortening (128.1 ± 4.6% vs. basal; p˂0.05) and accelerated relaxation (time to 50% relengthening: 49.2 ± 2.0% vs. basal; p˂0.05), effects abrogated by inhibition of: AKT with MK-2206, NO production with the NO synthase (NOS) inhibitor L-NAME and the specific NOS1 inhibitor nitroguanidine (NG), and CaMKII with KN-93. In agreement, an increase in NO in response to IGF-1 (133.8 ± 2.2%) was detected and prevented by both L-NAME and NG but not KN-93, suggesting that CaMKII activation was downstream NO. In addition, we determined CaMKII activity (P-CaMKII) and phosphorylation of its target, Thr17-PLN. IGF-1, by a NO-dependent mechanism, significantly increased both (227.2 ± 29.4% and 145.3 ± 5.4%, respectively) while no changes in the CaMKII phosphorylation site of ryanodine receptor were evident. The improvement in contractility induced by IGF-1 was associated with increased Ca2 + transient amplitude, rate of decay and SR content. Interestingly, this response was absent in cardiomyocytes from transgenic mice that express a CaMKII inhibitory peptide (AC3-I strain). Moreover, AC3-I mice subjected to swim training did develop physiological cardiac hypertrophy but not the contractile adaptation.Therefore, we conclude that NO-dependent CaMKII activation plays a critical role in the improvement in contractility induced by IGF-1 and exercise training. Interestingly, this pathway would not contribute to the adaptive hypertrophy.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Igf-1
dc.subject
Nitric Oxide Synthase 1
dc.subject
Camkii
dc.subject
Cardiac Contractility
dc.subject.classification
Fisiología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
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Sistemas Cardíaco y Cardiovascular
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Medicina Clínica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Nitric oxide and CaMKII: critical steps in the cardiac contractile response To IGF-1 and swim training
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-08T14:27:54Z
dc.journal.volume
112
dc.journal.pagination
16-26
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Burgos, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; Argentina
dc.description.fil
Fil: Yeves, Alejandra del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; Argentina
dc.description.fil
Fil: Barrena, Jorge P.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Vila Petroff, Martin Gerarde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; Argentina
dc.description.fil
Fil: Ennis, Irene Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones Cardiovasculares ; Argentina
dc.journal.title
Journal of Molecular and Cellular Cardiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.jmmc-online.com/article/S0022-2828(17)30292-4/fulltext
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.yjmcc.2017.08.014


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