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dc.contributor.author
Lopez de Armentia, María Milagros  
dc.contributor.author
Gaurón, María Celeste  
dc.contributor.author
Colombo, Maria Isabel  
dc.date.available
2018-06-19T19:59:03Z  
dc.date.issued
2017-10  
dc.identifier.citation
Lopez de Armentia, María Milagros; Gaurón, María Celeste; Colombo, Maria Isabel; Staphylococcus aureus alpha-toxin induces the formation of dynamic tubules labeled with LC3 within host cells in a Rab7 and rab1b-dependent manner; Frontiers Research Foundation; Frontiers in Cellular and Infection Microbiology; 7; 431; 10-2017; 1-17  
dc.identifier.issn
2235-2988  
dc.identifier.uri
http://hdl.handle.net/11336/49433  
dc.description.abstract
Staphylococcus aureus is a pathogen that causes severe infectious diseases that eventually lead to septic and toxic shock. S. aureus infection is characterized by the production of virulence factors, including enzymes and toxins. After internalization S. aureus resides in a phagosome labeled with Rab7 protein. Here, we show that S. aureus generates tubular structures marked with the small GTPases Rab1b and Rab7 and by the autophagic protein LC3 at early times post-infection. As shown by live cell imaging these tubular structures are highly dynamic, extend, branch and grow in length. We have named them S. aureus induced filaments (Saf). Furthermore, we demonstrate that the formation of these filaments depends on the integrity of microtubules and the activity of the motor protein Kinesin-1 (Kif5B) and the Rab-interacting lysosomal protein (RILP). Our group has previously reported that a-hemolysin, a secreted toxin of S. aureus, is responsible of the activation of the autophagic pathway induced by the bacteria. In the present report, we demonstrate that the autophagic protein LC3 is recruited to the membrane of S. aureus induced filaments and that a-hemolysin is the toxin that induces Saf formation. Interestingly, increasing the levels of intracellular cAMP significantly inhibited Saf biogenesis. Remarkably in this report we show the formation of tubular structures that emerge from the S. aureus-containing phagosome and that these tubules generation seems to be required for efficient bacteria replication.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Frontiers Research Foundation  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Autophagy  
dc.subject
Bacteria-Induced Tubules  
dc.subject
Bacterial Pathogens  
dc.subject
Intracellular Bacteria  
dc.subject
Staphylococcus Aureus  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Staphylococcus aureus alpha-toxin induces the formation of dynamic tubules labeled with LC3 within host cells in a Rab7 and rab1b-dependent manner  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-06-13T16:54:42Z  
dc.journal.volume
7  
dc.journal.number
431  
dc.journal.pagination
1-17  
dc.journal.pais
Suecia  
dc.journal.ciudad
Lausanne  
dc.description.fil
Fil: Lopez de Armentia, María Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina  
dc.description.fil
Fil: Gaurón, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina  
dc.description.fil
Fil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina  
dc.journal.title
Frontiers in Cellular and Infection Microbiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fcimb.2017.00431/full  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.3389/fcimb.2017.00431