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dc.contributor.author
Mazzei, Luciana Jorgelina
dc.contributor.author
García, Isabel Mercedes
dc.contributor.author
Calvo, Juan Pablo
dc.contributor.author
Casarotto, Mariana
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Fornes, Miguel Walter
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Abud, María Angélica
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Cuello Carrión, Fernando Darío
dc.contributor.author
Ferder, León
dc.contributor.author
Manucha, Walter Ariel Fernando
dc.date.available
2018-06-19T18:43:04Z
dc.date.issued
2016-03
dc.identifier.citation
Mazzei, Luciana Jorgelina; García, Isabel Mercedes; Calvo, Juan Pablo; Casarotto, Mariana; Fornes, Miguel Walter; et al.; Changes in renal WT-1 expression preceding hypertension development; BioMed Central; BMC Nephrology; 17; 34; 3-2016; 1-14
dc.identifier.issn
1471-2369
dc.identifier.uri
http://hdl.handle.net/11336/49382
dc.description.abstract
Background: Hypertension is a public health problem with mostly unknown causes, and where strong hereditary genetic alterations have not been fully elucidated. However, the use of experimental models has provided valuable information. Recent evidences suggest that alterations in key nephrogenic factors, such as Wilms' tumor 1 transcription factor (WT-1), could contribute to the development of hypertension. The aim of this paper is to evaluate the expression of WT-1 and related genes in the nephrogenic process in connection with the development of hypertension as well as the corresponding anatomical and functional correlation. Methods: Male spontaneously hypertensive and control rats were evaluated weekly from birth until week 8 of life. Their blood pressure was taken weekly using the tail-cuff blood pressure system. Weekly, 5 rats per group were sacrificed with a lethal injection of pentobarbital, and their kidneys were removed, decapsulated and weighed. The serum was collected for measuring biochemical parameters. The results were assessed using one-way analysis of variance for comparisons between groups. Results: The relationship between renal weight/total body weights was established, without significantly different values. These data were compared with apoptosis, fibrosis, number and size of the glomeruli. The elevation of systolic blood pressure was significant since week 6. Biochemical values differed slightly. Histology showed a slight increase in deposits of collagen fibers since week 4. Additionally, in kidney cortices, the expression of WT-1, heat shock protein 70 (Hsp70) and vitamin D receptors (VDR) decreased since week 4. Finally, we demonstrated ultrastructural damage to mitochondria since week 4. Conclusions: Our results would suggest an unprecedented link, possibly a regulatory mechanism, between WT-1 on nephrogenic alteration processes and their relationship with hypertension. Moreover, and previous to the increase in blood pressure, we demonstrated low expressions of WT-1, VDR and Hsp70 in kidneys from neonatal SHRs. If so, this may suggest that deregulation in the expression of WT-1 and its impact on nephrogenesis induction could be crucial in understanding the development and maintenance of hypertension.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Heat Shock Protein 70
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Hypertension
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Mitochondria
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Nephrogenesis
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Vitamin D Receptor
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Wilms' Tumor 1 Factors
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Patología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Changes in renal WT-1 expression preceding hypertension development
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-13T16:53:39Z
dc.journal.volume
17
dc.journal.number
34
dc.journal.pagination
1-14
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
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Fil: García, Isabel Mercedes. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
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Fil: Calvo, Juan Pablo. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
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Fil: Casarotto, Mariana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
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Fil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Abud, María Angélica. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
dc.description.fil
Fil: Cuello Carrión, Fernando Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Ferder, León. Universidad de Puerto Rico; Puerto Rico
dc.description.fil
Fil: Manucha, Walter Ariel Fernando. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.journal.title
BMC Nephrology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bmcnephrol.biomedcentral.com/articles/10.1186/s12882-016-0250-6
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s12882-016-0250-6
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