Artículo
Development of a dual vaccine for prevention of Brucella abortus infection and Escherichia coli O157: H7 intestinal colonization
Fecha de publicación:
03/2015
Editorial:
Elsevier
Revista:
Vaccine
ISSN:
0264-410X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Zoonoses that affect human and animal health have an important economic impact. In the study now presented, a bivalent vaccine has been developed that has the potential for preventing the transmission from cattle to humans of two bacterial pathogens: Brucella abortus and Shiga toxin-producing Escherichia coli (STEC). A 66kDa chimeric antigen, composed by EspA, Intimin, Tir, and H7 flagellin (EITH7) from STEC, was constructed and expressed in B. abortus δpgm vaccine strain (Babδpgm). Mice orally immunized with Babδpgm(EITH7) elicited an immune response with the induction of anti-EITH7 antibodies (IgA) that clears an intestinal infection of E. coli O157:H7 three times faster (t=4 days) than mice immunized with Babδpgm carrier strain (t=12 days). As expected, mice immunized with Babδpgm(EITH7) strain also elicited a protective immune response against B. abortus infection. A Brucella-based vaccine platform is described capable of eliciting a combined protective immune response against two bacterial pathogens with diverse lifestyles-the intracellular pathogen B. abortus and the intestinal extracellular pathogen STEC.
Palabras clave:
Brucella
,
Recombinant Vaccine
,
Shiga Toxin-Producing Escherichia Coli
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Citación
Iannino, Florencia; Herrmann, Claudia Karina; Roset, Mara Sabrina; Briones, Carlos Gabriel; Development of a dual vaccine for prevention of Brucella abortus infection and Escherichia coli O157: H7 intestinal colonization; Elsevier; Vaccine; 33; 19; 3-2015; 2248-2253
Compartir
Altmétricas