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dc.contributor.author
Yu, Haijie
dc.contributor.author
Benitez, Sergio Gonzalo
dc.contributor.author
Jung, Seung-Ryoung
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Kruse, Martin
dc.contributor.author
Seo, Jong Bae
dc.contributor.author
Koh, Duk-Su
dc.contributor.author
Muñoz, Estela Maris
dc.contributor.author
Hille, Bertil
dc.date.available
2018-06-19T18:09:10Z
dc.date.issued
2016-08
dc.identifier.citation
Yu, Haijie; Benitez, Sergio Gonzalo; Jung, Seung-Ryoung; Kruse, Martin; Seo, Jong Bae; et al.; GABAergic signaling in the rat pineal gland; Wiley Blackwell Publishing, Inc; Journal of Pineal Research; 61; 1; 8-2016; 69-81
dc.identifier.issn
0742-3098
dc.identifier.uri
http://hdl.handle.net/11336/49353
dc.description.abstract
Pinealocytes secrete melatonin at night in response to norepinephrine released from sympathetic nerve terminals in the pineal gland. The gland also contains many other neurotransmitters whose cellular disposition, activity, and relevance to pineal function are not understood. Here, we clarify sources and demonstrate cellular actions of the neurotransmitter γ-aminobutyric acid (GABA) using Western blotting and immunohistochemistry of the gland and electrical recording from pinealocytes. GABAergic cells and nerve fibers, defined as containing GABA and the synthetic GAD67, were identified. The cells represent a subset of interstitial cells while the nerve fibers were distinct from the sympathetic innervation. The GABAA receptor subunit α1 was visualized in close proximity of both GABAergic and sympathetic nerve fibers as well as fine extensions among pinealocytes and blood vessels. The GABAB 1 receptor subunit was localized in the interstitial compartment but not in pinealocytes. Electrophysiology of isolated pinealocytes revealed that GABA and muscimol elicit strong inward chloride currents sensitive to bicuculline and picrotoxin, clear evidence for functional GABAA receptors on the surface membrane. Applications of elevated potassium solution or the neurotransmitter acetylcholine depolarized the pinealocyte membrane potential enough to open voltage-gated Ca2+ channels leading to intracellular calcium elevations. GABA repolarized the membrane and shut off such calcium rises. In 48–72-h cultured intact glands, GABA application neither triggered melatonin secretion by itself nor affected norepinephrine-induced secretion. Thus, strong elements of GABA signaling are present in pineal glands that make large electrical responses in pinealocytes, but physiological roles need to be found.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Bicuculline
dc.subject
Gaba
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Gabaa Receptor
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Gad67
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Melatonin Secretion
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Membrane Potential
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Pineal Gland
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
GABAergic signaling in the rat pineal gland
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-13T16:53:09Z
dc.journal.volume
61
dc.journal.number
1
dc.journal.pagination
69-81
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Yu, Haijie. University of Washington; Estados Unidos
dc.description.fil
Fil: Benitez, Sergio Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Jung, Seung-Ryoung. University of Washington; Estados Unidos
dc.description.fil
Fil: Kruse, Martin. University of Washington; Estados Unidos
dc.description.fil
Fil: Seo, Jong Bae. University of Washington; Estados Unidos
dc.description.fil
Fil: Koh, Duk-Su. University of Washington; Estados Unidos
dc.description.fil
Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Hille, Bertil. University of Washington; Estados Unidos
dc.journal.title
Journal of Pineal Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12328
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/jpi.12328
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489258/
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