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dc.contributor.author
Losinno, Antonella Denise
dc.contributor.author
Sorrivas, Viviana
dc.contributor.author
Ezquer, Eduardo Marcelo
dc.contributor.author
Ezquer, Fernando
dc.contributor.author
Lopez, Luis Alberto
dc.contributor.author
Morales, Alfonsina
dc.date.available
2018-06-19T17:58:21Z
dc.date.issued
2016-08
dc.identifier.citation
Losinno, Antonella Denise; Sorrivas, Viviana; Ezquer, Eduardo Marcelo; Ezquer, Fernando; Lopez, Luis Alberto; et al.; Changes of myoid and endothelial cells in the peritubular wall during contraction of the seminiferous tubule; Springer; Cell and Tissue Research; 365; 2; 8-2016; 425-435
dc.identifier.issn
0302-766X
dc.identifier.uri
http://hdl.handle.net/11336/49340
dc.description.abstract
The wall of the seminiferous tubule in rodents consists of an inner layer of myoid cells covered by an outer layer of endothelial cells. Myoid cells are a type of smooth muscle cell containing α-actin filaments arranged in two independent layers that contract when stimulated by endothelin-1. The irregular surface relief of the tubular wall is often considered a hallmark of contraction induced by a variety of stimuli. We examine morphological changes of the rat seminiferous tubule wall during contraction by a combination of light, confocal, transmission and scanning electron microscopy. During ET-1-induced contraction, myoid cells changed from a flat to a conical shape, but their actin filaments remained in independent layers. As a consequence of myoid cell contraction, the basement membrane became wavy, orientation of collagen fibers in the extracellular matrix was altered and the endothelial cell layer became folded. To observe the basement of the myoid cell cone, the endothelial cell monolayer was removed by collagenase digestion prior to SEM study. In contracted tubules, it is possible to distinguish cell relief: myoid cells have large folds on the external surface oriented parallel to the tubular axis, whereas endothelial cells have numerous cytoplasmic projections facing the interstitium. The myoid cell cytoskeleton is unusual in that the actin filaments are arranged in two orthogonal layers, which adopt differing shapes during contraction with myoid cells becoming cone-shaped. This arrangement impacts on other components of the seminiferous tubule wall and affects the propulsion of the tubular contents to the rete testis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Alpha-Actin
dc.subject
Endothelin-1
dc.subject
Peritubular Myoid Cell
dc.subject
Seminiferous Tubules
dc.subject
Testis Ultrastructure
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Changes of myoid and endothelial cells in the peritubular wall during contraction of the seminiferous tubule
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-13T16:52:51Z
dc.journal.volume
365
dc.journal.number
2
dc.journal.pagination
425-435
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Losinno, Antonella Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Sorrivas, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
dc.description.fil
Fil: Ezquer, Eduardo Marcelo. Universidad del Desarrollo; Chile
dc.description.fil
Fil: Ezquer, Fernando. Universidad del Desarrollo; Chile
dc.description.fil
Fil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Morales, Alfonsina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.journal.title
Cell and Tissue Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00441-016-2386-x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00441-016-2386-x


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