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dc.contributor.author
Harizanova, Jana
dc.contributor.author
Fermin, Yessica
dc.contributor.author
Malik-Sheriff, Rahuman S.
dc.contributor.author
Wieczorek, Jakob
dc.contributor.author
Ickstadt, Katja
dc.contributor.author
Grecco, Hernan Edgardo
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dc.contributor.author
Zamir, Eli
dc.date.available
2018-06-19T15:41:54Z
dc.date.issued
2016-08
dc.identifier.citation
Harizanova, Jana; Fermin, Yessica; Malik-Sheriff, Rahuman S.; Wieczorek, Jakob; Ickstadt, Katja; et al.; Highly multiplexed imaging uncovers changes in compositional noise within assembling focal adhesions; Public Library of Science; Plos One; 11; 8; 8-2016; 1-18
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/49270
dc.description.abstract
Integrin adhesome proteins bind each other in alternative manners, forming within the cell diverse cell-matrix adhesion sites with distinct properties. An intriguing question is how such modular assembly of adhesion sites is achieved correctly solely by self-organization of their components. Here we address this question using high-throughput multiplexed imaging of eight proteins and two phosphorylation sites in a large number of single focal adhesions.We found that during the assembly of focal adhesions the variances of protein densities decrease while the correlations between them increase, suggesting reduction in the noise levels within these structures. These changes correlate independently with the area and internal density of focal adhesions, but not with their age or shape. Artificial neural network analysis indicates that a joint consideration of multiple components improves the predictability of paxillin and zyxin levels in internally dense focal adhesions. This suggests that paxillin and zyxin densities in focal adhesions are fine-tuned by integrating the levels of multiple other components, thus averaging-out stochastic fluctuations. Based on these results we propose that increase in internal protein densities facilitates noise suppression in focal adhesions, while noise suppression enables their stable growth and further density increase - hence forming a feedback loop giving rise to a quality-controlled assembly.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Focal Adhesion
dc.subject
Multidimensional Imaging
dc.subject
Noise
dc.subject.classification
Otras Ciencias Biológicas
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Highly multiplexed imaging uncovers changes in compositional noise within assembling focal adhesions
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-12T16:09:43Z
dc.journal.volume
11
dc.journal.number
8
dc.journal.pagination
1-18
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Harizanova, Jana. Administrative Headquarters Of The Max Planck Society;
dc.description.fil
Fil: Fermin, Yessica. Universitat Dortmund; Alemania
dc.description.fil
Fil: Malik-Sheriff, Rahuman S.. European Bioinformatics Institute; . Administrative Headquarters Of The Max Planck Society; . Imperial College London; Reino Unido
dc.description.fil
Fil: Wieczorek, Jakob. Universitat Dortmund; Alemania
dc.description.fil
Fil: Ickstadt, Katja. Universitat Dortmund; Alemania
dc.description.fil
Fil: Grecco, Hernan Edgardo. Administrative Headquarters Of The Max Planck Society; . Universidad de Buenos Aires. Facultad de Ciencias Exactas. Departamento de Ecología, Genética y Evolución. Buenos Aires; Argentina
dc.description.fil
Fil: Zamir, Eli. Administrative Headquarters Of The Max Planck Society;
dc.journal.title
Plos One
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0160591
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