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dc.contributor.author
Missirlis, Dimitris
dc.contributor.author
Haraszti, Tamás
dc.contributor.author
Scheele, Catharina v. C.
dc.contributor.author
Wiegand, Tina
dc.contributor.author
Diaz, Carolina
dc.contributor.author
Neubauer, Stefanie
dc.contributor.author
Rechenmacher, Florian
dc.contributor.author
Kessler, Horst
dc.contributor.author
Spatz, Joachim P.
dc.date.available
2018-06-18T18:42:10Z
dc.date.issued
2016-03
dc.identifier.citation
Missirlis, Dimitris; Haraszti, Tamás; Scheele, Catharina v. C.; Wiegand, Tina; Diaz, Carolina; et al.; Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin; Nature Publishing Group; Scientific Reports; 6; 3-2016; 1-18; 23258
dc.identifier.issn
2045-2322
dc.identifier.uri
http://hdl.handle.net/11336/49069
dc.description.abstract
The interplay between specific integrin-mediated matrix adhesion and directional persistence in cell migration is not well understood. Here, we characterized fibroblast adhesion and migration on the extracellular matrix glycoproteins fibronectin and vitronectin, focusing on the role of α5 β1 and αv β3 integrins. Fibroblasts manifested high directional persistence in migration on fibronectin-, but not vitronectin-coated substrates, in a ligand density-dependent manner. Fibronectin stimulated α5 β1-dependent organization of the actin cytoskeleton into oriented, ventral stress fibers, and assembly of dynamic, polarized protrusions, characterized as regions free of stress fibers and rich in nascent adhesions at their edge. Such protrusions correlated with persistent, local leading edge advancement, but were not sufficient, nor necessary for directional migration over longer times. Selective blocking of αv β3 or α5 β1 integrins using small molecule integrin antagonists reduced directional persistence on fibronectin, indicating integrin cooperativity in maintaining directionality. On the other hand, patterned substrates, designed to selectively engage either integrin, or their combination, were not sufficient to establish directional migration. Overall, our study demonstrates adhesive coating-dependent regulation of directional persistence in fibroblast migration and challenges the generality of the previously suggested role of β1 and β3 integrins in directional migration.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Focal Adhesion
dc.subject
Integrin Signaling
dc.subject
Fibronectin
dc.subject
Migration
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Substrate engagement of integrins α5 β1 and αv β3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-14T19:05:43Z
dc.journal.volume
6
dc.journal.pagination
1-18; 23258
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Missirlis, Dimitris. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
dc.description.fil
Fil: Haraszti, Tamás. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
dc.description.fil
Fil: Scheele, Catharina v. C.. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
dc.description.fil
Fil: Wiegand, Tina. University of Heidelberg; Alemania. Max Planck Institute for Intelligent Systems; Alemania
dc.description.fil
Fil: Diaz, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Max Planck Institute for Intelligent Systems; Alemania. University of Heidelberg; Alemania
dc.description.fil
Fil: Neubauer, Stefanie. Technische Universitat Munchen; Alemania
dc.description.fil
Fil: Rechenmacher, Florian. Technische Universitat Munchen; Alemania
dc.description.fil
Fil: Kessler, Horst. Technische Universitat Munchen; Alemania
dc.description.fil
Fil: Spatz, Joachim P.. University of Heidelberg; Alemania. Max Planck Institute for Intelligent Systems; Alemania
dc.journal.title
Scientific Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/srep23258
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep23258


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