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dc.contributor.author
Vazquez, Romina Florencia  
dc.contributor.author
Maté, Sabina María  
dc.contributor.author
Bakas, Laura Susana  
dc.contributor.author
Muñoz Garay, Carlos  
dc.contributor.author
Herlax, Vanesa Silvana  
dc.date.available
2018-06-18T15:43:05Z  
dc.date.issued
2016-08  
dc.identifier.citation
Vazquez, Romina Florencia; Maté, Sabina María; Bakas, Laura Susana; Muñoz Garay, Carlos; Herlax, Vanesa Silvana; Relationship between intracellular calcium and morphologic changes in rabbit erythrocytes: Effects of the acylated and unacylated forms of E. coli alpha-hemolysin; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1858; 8; 8-2016; 1944-1953  
dc.identifier.issn
0005-2736  
dc.identifier.uri
http://hdl.handle.net/11336/48991  
dc.description.abstract
Alpha-hemolysin (HlyA) is a hemolytic and cytotoxic protein secreted by uropathogenic Escherichia coli strains, whose expression correlates with the severity of the infections produced. HlyA is synthesized as a protoxin, ProHlyA, that becomes matured to the active form in the cytosol by hemolysin-C-directed fatty acylation at the ε-amino residues of Lys 564 and Lys 690, before export from the toxin-producing bacteria. This posttranslational modification is remarkable because the nature of the protein is changed by the lipidic moiety from a benign protein to a frank toxin.In the present work, we demonstrated for the first time that, despite being hemolitically inactive, ProHlyA induced cellular morphologic changes in rabbit erythrocytes. A discocyte-to-echinocyte transformation was triggered by the protoxin in the absence of any accompanying increase in intracellular-Ca2+ levels. In addition, the Ca2+-influx kinetics in HlyA-treated erythrocytes indicated that the active toxin induced an elevation in intraerythrocyte-Ca2+ content in a biphasic manner, with an initial rise in Ca2+ that depended on the association of the protein with the target membrane and a second increment corresponding to an activation of purinergic channels. The first increase was sufficient to trigger a discocyte-echinocyte-spherocyte transition in erythrocyte shape, though the subsequent rise mediated by purinergic signalling was essential for the occurrence of hemolysis. The results presented here provide new insights into the mechanism of action of this toxin.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Rtx Toxins  
dc.subject
Hlya  
dc.subject
Calcium  
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Purinergic Receptor  
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Unacylated Protein  
dc.subject
Exovesicles  
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Discocyte-Echinocyte-Spherocyte Transition  
dc.subject.classification
Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Relationship between intracellular calcium and morphologic changes in rabbit erythrocytes: Effects of the acylated and unacylated forms of E. coli alpha-hemolysin  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-06-14T19:08:00Z  
dc.journal.volume
1858  
dc.journal.number
8  
dc.journal.pagination
1944-1953  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Vazquez, Romina Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
dc.description.fil
Fil: Maté, Sabina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
dc.description.fil
Fil: Bakas, Laura Susana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina  
dc.description.fil
Fil: Muñoz Garay, Carlos. Universidad Nacional Autónoma de México; México  
dc.description.fil
Fil: Herlax, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
dc.journal.title
Biochimica et Biophysica Acta - Biomembranes  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.bbamem.2016.05.013  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005273616301870