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dc.contributor.author
Von Bilderling, Catalina
dc.contributor.author
Caldarola, Martín
dc.contributor.author
Masip, Martín E.
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Bragas, Andrea Veronica
dc.contributor.author
Pietrasanta, Lia
dc.date.available
2018-06-18T15:42:54Z
dc.date.issued
2017-01
dc.identifier.citation
Von Bilderling, Catalina; Caldarola, Martín; Masip, Martín E.; Bragas, Andrea Veronica; Pietrasanta, Lia; Monitoring in real-time focal adhesion protein dynamics in response to a discrete mechanical stimulus; American Institute of Physics; Review of Scientific Instruments; 88; 1; 1-2017; 1-9
dc.identifier.issn
0034-6748
dc.identifier.uri
http://hdl.handle.net/11336/48990
dc.description.abstract
The adhesion of cells to the extracellular matrix is a hierarchical, force-dependent, multistage processthat evolves at several temporal scales. An understanding of this complex process requires a precisemeasurement of forces and its correlation with protein responses in living cells. We present a methodto quantitatively assess live cell responses to a local and specific mechanical stimulus. Our approachcombines atomic force microscopy with fluorescence imaging. Using this approach, we evaluatedthe recruitment of adhesion proteins such as vinculin, focal adhesion kinase, paxillin, and zyxintriggered by applying forces in the nN regime to live cells. We observed in real time the developmentof nascent adhesion sites, evident from the accumulation of early adhesion proteins at the positionwhere the force was applied. We show that the method can be used to quantify the recruitmentcharacteristic times for adhesion proteins in the formation of focal complexes. We also found aspatial remodeling of the mature focal adhesion protein zyxin as a function of the applied force.Our approach allows the study of a variety of complex biological processes involved in cellularmechanotransduction
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Institute of Physics
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Focal Adhesions
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Mechanotransduction
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Optical Microscope
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Afm
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Astronomía
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Ciencias Físicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Monitoring in real-time focal adhesion protein dynamics in response to a discrete mechanical stimulus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-12T16:09:12Z
dc.journal.volume
88
dc.journal.number
1
dc.journal.pagination
1-9
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Von Bilderling, Catalina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Caldarola, Martín. Leiden University; Países Bajos. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Masip, Martín E.. Universidad de Buenos Aires; Argentina. Institut Max Planck fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Bragas, Andrea Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Pietrasanta, Lia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Review of Scientific Instruments
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1063/1.4973664


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