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dc.contributor.author
Calderón Fernández, Gustavo Mario  
dc.contributor.author
Moriconi, Debora Elizabeth  
dc.contributor.author
Dulbecco, Andrea Belen  
dc.contributor.author
Juarez, Marta Patricia  
dc.date.available
2018-06-18T15:29:21Z  
dc.date.issued
2017-09  
dc.identifier.citation
Calderón Fernández, Gustavo Mario; Moriconi, Debora Elizabeth; Dulbecco, Andrea Belen; Juarez, Marta Patricia; Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument; Entomological Society of America; Journal of Medical Entomology; 54; 6; 9-2017; 1531-1542  
dc.identifier.issn
0022-2585  
dc.identifier.uri
http://hdl.handle.net/11336/48973  
dc.description.abstract
The insect integument, formed by the cuticle and the underlying epidermis, is essential for insect fitness, regulation of lipid biosynthesis and storage, insect growth and feeding, together with development progress. Its participation in insecticide resistance has also been outlined. Triatoma infestans Klug is one of the major vectors of Chagas disease in South America; however, genomic data is scarce. In this study, we performed a transcriptome analysis of the nymph integument in order to identify which genes are expressed and their putative role. Using the 454 GS-FLX sequencing platform, we obtained approximately 144620 reads from the integument tissue. These reads were assembled into 6495 isotigs and 8504 singletons. Based on BLAST similarity searches, about 8000 transcripts were annotated with known genes, conserved domains, and/or Gene Ontology (GO) terms. The most abundant transcripts corresponded to transcription factors and nucleic acid metabolism, membrane receptors, cell signaling, and proteins related to cytoskeleton, transport and cell energy processes, among others. More than 10% of the transcripts encoded proteins putatively involved in the metabolism of fatty acids and related components (fatty acid synthases, elongases, desaturases, fatty alcohol reductases), structural integument proteins, and the insecticide detoxification system (among them, cytochrome P450s, esterases and glutathione transferases). Real time qPCR assays were used to investigate their putative participation in the resistance mechanism. This preliminary study is the first transcriptome analysis of a triatomine integument, and together with prior biochemical information, will help further understand the role of the integument in a wide array of mechanisms  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Entomological Society of America  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Transcriptome  
dc.subject
Integument  
dc.subject
Insecticide Resistance  
dc.subject
Lipids  
dc.subject
Cuticle Proteins  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Transcriptome Analysis of the Triatoma infestans (Hemiptera: Reduviidae) Integument  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-06-15T18:35:36Z  
dc.journal.volume
54  
dc.journal.number
6  
dc.journal.pagination
1531-1542  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Lanham  
dc.description.fil
Fil: Calderón Fernández, Gustavo Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
dc.description.fil
Fil: Moriconi, Debora Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
dc.description.fil
Fil: Dulbecco, Andrea Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
dc.description.fil
Fil: Juarez, Marta Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
dc.journal.title
Journal of Medical Entomology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1093/jme/tjx151  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article-abstract/54/6/1531/4243555