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dc.contributor.author Pistone Creydt, Virginia
dc.contributor.author Fletcher, Sabrina Johanna
dc.contributor.author Giudice, Jimena
dc.contributor.author Bruzzone, Ariana
dc.contributor.author Chasseing, Norma Alejandra
dc.contributor.author Gonzalez, Gustavo Eduardo
dc.contributor.author Sacca, Paula Alejandra
dc.contributor.author Calvo, Juan Carlos
dc.date.available 2016-03-21T17:48:03Z
dc.date.issued 2012-07-19
dc.identifier.citation Pistone Creydt, Virginia; Fletcher, Sabrina Johanna; Giudice, Jimena; Bruzzone, Ariana; Chasseing, Norma Alejandra; et al.; Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells; Springer; Clinical & Translational Oncology; 15; 2; 19-7-2012; 124-131
dc.identifier.issn 1699-048X
dc.identifier.uri http://hdl.handle.net/11336/4879
dc.description.abstract Introduction: Stromal-epithelial interactions mediate both breast development and breast cancer progression. In the present work, we evaluated the effects of conditioned media (CMs) of human adipose tissue explants from normal (hATN) and tumor (hATT) breast on proliferation, adhesion, migration and metalloproteases (MMPs) activity on tumor (MCF-7 and IBH-7) and non-tumor (MCF-10A) human breast epithelial cell lines. Material and methods: Human adipose tissues were obtained from patients and the conditioned medium from hATN and hATT collected after 24 hours of incubation. MCF-10A, MCF-7 and IBH-7 cells were grown and incubated with CMs and proliferation and adhesion, as well as migration ability and metalloprotease activity, of epithelial cells after exposing cell cultures to hATN- or hATT-CMs were quantified. The statistical significance between different experimental conditions was evaluated by one-way ANOVA. Tukey´s post-hoc tests were performed. Results: Tumor and non-tumor breast epithelial cells significantly increased their proliferation activity after 24 hours of treatment with hATT-CMs compared to control-CMs. Furthermore, cellular adhesion of these two tumor cell lines was significantly lower with hATT-CMs than with hATN-CMs. Therefore, hATT-CMs seem to induce significantly lower expression or less activity of the components involved in cellular adhesion than hATN-CMs. In addition, hATT-CMs induced pro-MMP-9 and MMP-9 activity and increased the migration of MCF-7 and IBH-7 cells compared to hATN-CMs. Conclusions: We conclude that the microenvironment of the tumor interacts in a dynamic way with the mutated epithelium. This evidence leads to the possibility to modify the tumor behavior/phenotype through the regulation or modification of its microenvironment. We developed a model in which we obtained conditioned media from adipose tissue explants completely, either from normal or tumor breast. In this way, we studied the contribution of soluble factors independently of the possible effects of direct cell contact.
dc.format application/pdf
dc.language.iso eng
dc.publisher Springer
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ADIPOSE TISSUE
dc.subject BREAST CANCER
dc.subject STEM CELLS
dc.subject.classification Patología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-03-30 10:35:44.97925-03
dc.identifier.eissn 1699-3055
dc.journal.volume 15
dc.journal.number 2
dc.journal.pagination 124-131
dc.journal.pais Italia
dc.journal.ciudad Milán
dc.description.fil Fil: Pistone Creydt, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil Fil: Fletcher, Sabrina Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil Fil: Giudice, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil Fil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil Fil: Chasseing, Norma Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil Fil: Gonzalez, Gustavo Eduardo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Angel H. Roffo"; Argentina
dc.description.fil Fil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.journal.title Clinical & Translational Oncology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://doi.org/10.1007/s12094-012-0896-x
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs12094-012-0896-x
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12094-012-0896-x


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)