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dc.contributor.author Balabanidou, Vasileia
dc.contributor.author Kampouraki, Anastasia
dc.contributor.author Mac Lean, Marina
dc.contributor.author Blomquist, Gary J.
dc.contributor.author Tittiger, Claus
dc.contributor.author Juarez, Marta Patricia
dc.contributor.author Mijailovsky, Sergio Javier
dc.contributor.author Chalepakis, George
dc.contributor.author Anthousi, Amalia
dc.contributor.author Lynd, Amy
dc.contributor.author Antoine, Sanou
dc.contributor.author Hemingway, Janet
dc.contributor.author Ranson, Hilary
dc.contributor.author Lycett, Gareth J.
dc.contributor.author Vontas, John
dc.date.available 2018-06-15T14:58:41Z
dc.date.issued 2016-07
dc.identifier.citation Balabanidou, Vasileia ; Kampouraki, Anastasia; Mac Lean, Marina; Blomquist, Gary J. ; Tittiger, Claus; et al.; Cytochrome P450associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae.; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 113; 33; 7-2016; 9268-9273
dc.identifier.issn 0027-8424
dc.identifier.uri http://hdl.handle.net/11336/48776
dc.description.abstract The role of cuticle changes in insecticide resistance in the major malaria vector Anopheles gambiae was assessed. The rate of internalization of 14C deltamethrin was significantly slower in a resistant strain than in a susceptible strain. Topical application of an acetone insecticide formulation to circumvent lipid-based uptake barriers decreased the resistance ratio by ∼50%. Cuticle analysis by electron microscopy and characterization of lipid extracts indicated that resistant mosquitoes had a thicker epicuticular layer and a significant increase in cuticular hydrocarbon (CHC) content (∼29%). However, the CHC profile and relative distribution were similar in resistant and susceptible insects. The cellular localization and in vitro activity of two P450 enzymes, CYP4G16 and CYP4G17, whose genes are frequently overexpressed in resistant Anopheles mosquitoes, were analyzed. These enzymes are potential orthologs of the CYP4G1/2 enzymes that catalyze the final step of CHC biosynthesis in Drosophila and Musca domestica, respectively. Immunostaining indicated that both CYP4G16 and CYP4G17 are highly abundant in oenocytes, the insect cell type thought to secrete hydrocarbons. However, an intriguing difference was indicated; CYP4G17 occurs throughout the cell, as expected for a microsomal P450, but CYP4G16 localizes to the periphery of the cell and lies on the cytoplasmic side of the cell membrane, a unique position for a P450 enzyme. CYP4G16 and CYP4G17 were functionally expressed in insect cells. CYP4G16 produced hydrocarbons from a C18 aldehyde substrate and thus has bona fide decarbonylase activity similar to that of dmCYP4G1/2. The data support the hypothesis that the coevolution of multiple mechanisms, including cuticular barriers, has occurred in highly pyrethroid-resistant An. gambiae.
dc.format application/pdf
dc.language.iso eng
dc.publisher National Academy of Sciences
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject MALARIA
dc.subject INSECTICIDE RESISTANCE
dc.subject HYDROCARBONS
dc.subject MOSQUITO CUTICLE
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Cytochrome P450associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae.
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-06-14T19:06:10Z
dc.journal.volume 113
dc.journal.number 33
dc.journal.pagination 9268-9273
dc.journal.pais Estados Unidos
dc.journal.ciudad Washington
dc.description.fil Fil: Balabanidou, Vasileia. Foundation for Research and Technology-Hellas; Grecia. Universidad de Creta; Grecia
dc.description.fil Fil: Kampouraki, Anastasia. Universidad de Creta; Grecia
dc.description.fil Fil: Mac Lean, Marina. University of Nevada; Estados Unidos
dc.description.fil Fil: Blomquist, Gary J.. University of Nevada; Estados Unidos
dc.description.fil Fil: Tittiger, Claus. University of Nevada; Estados Unidos
dc.description.fil Fil: Juarez, Marta Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil Fil: Mijailovsky, Sergio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil Fil: Chalepakis, George. Universidad de Creta; Grecia
dc.description.fil Fil: Anthousi, Amalia. Universidad de Creta; Grecia
dc.description.fil Fil: Lynd, Amy. Liverpool School of Tropical Medicine; Reino Unido
dc.description.fil Fil: Antoine, Sanou. Liverpool School of Tropical Medicine; Reino Unido
dc.description.fil Fil: Hemingway, Janet. Liverpool School of Tropical Medicine; Reino Unido
dc.description.fil Fil: Ranson, Hilary. Liverpool School of Tropical Medicine; Reino Unido
dc.description.fil Fil: Lycett, Gareth J.. Liverpool School of Tropical Medicine; Reino Unido
dc.description.fil Fil: Vontas, John. Foundation for Research and Technology-Hellas; Grecia. Agricultural University of Athens; Grecia
dc.journal.title Proceedings of the National Academy of Sciences of The United States of America
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1073/pnas.1608295113
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/113/33/9268
dc.conicet.fuente individual


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)