Mostrar el registro sencillo del ítem
dc.contributor.author
Spaiardi, Paolo
dc.contributor.author
Tavazzani, Elisa
dc.contributor.author
Manca, Marco
dc.contributor.author
Milesi, Verónica
dc.contributor.author
Russo, Giancarlo
dc.contributor.author
Prigioni, Ivo
dc.contributor.author
Marcotti, Walter
dc.contributor.author
Magistretti, Jacopo
dc.contributor.author
Masetto, Sergio
dc.date.available
2018-06-14T21:37:42Z
dc.date.issued
2017-11
dc.identifier.citation
Spaiardi, Paolo; Tavazzani, Elisa; Manca, Marco; Milesi, Verónica; Russo, Giancarlo; et al.; An allosteric gating model recapitulates the biophysical properties of IK,L expressed in mouse vestibular type I hair cells; Wiley Blackwell Publishing, Inc; The Journal Of Physiology; 595; 21; 11-2017; 6735-6750
dc.identifier.issn
0022-3751
dc.identifier.uri
http://hdl.handle.net/11336/48729
dc.description.abstract
Type I and type II hair cells are the sensory receptors of the mammalian vestibular epithelia. Type I hair cells are characterized by their basolateral membrane being enveloped in a single large afferent nerve terminal, named the calyx, and by the expression of a low‐voltage‐activated outward rectifying K+ current, IK,L. The biophysical properties and molecular profile of IK,L are still largely unknown. By using the patch‐clamp whole‐cell technique, we examined the voltage‐ and time‐dependent properties of IK,L in type I hair cells of the mouse semicircular canal. We found that the biophysical properties of IK,L were affected by an unstable K+ equilibrium potential (VeqK+). Both the outward and inward K+ currents shifted VeqK+ consistent with K+ accumulation or depletion, respectively, in the extracellular space, which we attributed to a residual calyx attached to the basolateral membrane of the hair cells. We therefore optimized the hair cell dissociation protocol in order to isolate mature type I hair cells without their calyx. In these cells, the uncontaminated IK,L showed a half‐activation at –79.6 mV and a steep voltage dependence (2.8 mV). IK,L also showed complex activation and deactivation kinetics, which we faithfully reproduced by an allosteric channel gating scheme where the channel is able to open from all (five) closed states. The ‘early’ open states substantially contribute to IK,L activation at negative voltages. This study provides the first complete description of the ‘native’ biophysical properties of IK,L in adult mouse vestibular type I hair cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Channel Gating Model
dc.subject
Ik,L
dc.subject
Type I Vestibular Hair Cell
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
An allosteric gating model recapitulates the biophysical properties of IK,L expressed in mouse vestibular type I hair cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-13T14:19:40Z
dc.journal.volume
595
dc.journal.number
21
dc.journal.pagination
6735-6750
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Spaiardi, Paolo. Università degli Studi di Pavia; Italia
dc.description.fil
Fil: Tavazzani, Elisa. Università degli Studi di Pavia; Italia
dc.description.fil
Fil: Manca, Marco. Università degli Studi di Pavia; Italia
dc.description.fil
Fil: Milesi, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
dc.description.fil
Fil: Russo, Giancarlo. Università degli Studi di Pavia; Italia
dc.description.fil
Fil: Prigioni, Ivo. Università degli Studi di Pavia; Italia
dc.description.fil
Fil: Marcotti, Walter. University of Sheffield. Department of Biomedical Science; Reino Unido
dc.description.fil
Fil: Magistretti, Jacopo. Università degli Studi di Pavia; Italia
dc.description.fil
Fil: Masetto, Sergio. Università degli Studi di Pavia; Italia
dc.journal.title
The Journal Of Physiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://physoc.onlinelibrary.wiley.com/doi/abs/10.1113/JP274202
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1113/JP274202
Archivos asociados