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dc.contributor.author
Baccarini, Leticia Cecilia  
dc.contributor.author
Martínez Montañés, Fernando  
dc.contributor.author
Rossi, Silvia Graciela  
dc.contributor.author
Proft, Markus  
dc.contributor.author
Portela, Paula  
dc.date.available
2018-06-14T19:31:52Z  
dc.date.issued
2015-11  
dc.identifier.citation
Baccarini, Leticia Cecilia; Martínez Montañés, Fernando; Rossi, Silvia Graciela; Proft, Markus; Portela, Paula; PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae; Elsevier Science; Biochimica Et Biophysica Acta-gene Regulatory Mechanisms; 1849; 11; 11-2015; 1329-1339  
dc.identifier.issn
1874-9399  
dc.identifier.uri
http://hdl.handle.net/11336/48697  
dc.description.abstract
Gene expression regulation by intracellular stimulus-activated protein kinases is essential for cell adaptation to environmental changes. There are three PKA catalytic subunits in Saccharomyces cerevisiae: Tpk1, Tpk2, and Tpk3 and one regulatory subunit: Bcy1. Previously, it has been demonstrated that Tpk1 and Tpk2 are associated with coding regions and promoters of target genes in a carbon source and oxidative stress dependent manner. Here we studied five genes, ALD6, SED1, HSP42, RPS29B, and RPL1B whose expression is regulated by saline stress. We found that PKA catalytic and regulatory subunits are associated with both coding regions and promoters of the analyzed genes in a stress dependent manner. Tpk1 and Tpk2 recruitment was completely abolished in catalytic inactive mutants. BCY1 deletion changed the binding kinetic to chromatin of each Tpk isoform and this strain displayed a deregulated gene expression in response to osmotic stress. In addition, yeast mutants with high PKA activity exhibit sustained association to target genes of chromatin-remodeling complexes such as Snf2-catalytic subunit of the SWI/SNF complex and Arp8-component of INO80 complex, leading to upregulation of gene expression during osmotic stress. Tpk1 accumulation in the nucleus was stimulated upon osmotic stress, while the nuclear localization of Tpk2 and Bcy1 showed no change. We found that each PKA subunit is transported into the nucleus by a different β-karyopherin pathway. Moreover, β-karyopherin mutant strains abolished the chromatin association of Tpk1 or Tpk2, suggesting that nuclear localization of PKA catalytic subunits is required for its association to target genes and properly gene expression.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Chromatin  
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Osmotic Stress  
dc.subject
Pka  
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Saccharomyces Cerevisiae  
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Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-06-07T20:02:39Z  
dc.identifier.eissn
1876-4320  
dc.journal.volume
1849  
dc.journal.number
11  
dc.journal.pagination
1329-1339  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Baccarini, Leticia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina  
dc.description.fil
Fil: Martínez Montañés, Fernando. University of Fribourg; Suiza  
dc.description.fil
Fil: Rossi, Silvia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina  
dc.description.fil
Fil: Proft, Markus. Instituto de Biomedicina; España. Consejo Superior de Investigaciones Científicas; España  
dc.description.fil
Fil: Portela, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina  
dc.journal.title
Biochimica Et Biophysica Acta-gene Regulatory Mechanisms  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1874939915001935  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.bbagrm.2015.09.007