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dc.contributor.author
Mecchia, Martin Alejandro
dc.contributor.author
Debernardi, Juan Manuel
dc.contributor.author
Rodriguez Virasoro, Ramiro Esteban
dc.contributor.author
Schommer, Carla
dc.contributor.author
Palatnik, Javier Fernando
dc.date.available
2016-03-18T18:31:47Z
dc.date.issued
2013-01
dc.identifier.citation
Mecchia, Martin Alejandro; Debernardi, Juan Manuel; Rodriguez Virasoro, Ramiro Esteban; Schommer, Carla; Palatnik, Javier Fernando; MicroRNA miR396 and RDR6 synergistically regulate leaf development; Elsevier; Mechanisms of Development; 113; 1; 1-2013; 2-13
dc.identifier.issn
0925-4773
dc.identifier.uri
http://hdl.handle.net/11336/4862
dc.description.abstract
The miroRNA (miRNA) miR396 regulates Growth-Regulating Factors (GRFs), a plant specific family of transcription factors. Overexpression of miR396 causes a decrease in the GRFs that has been shown to affect cell proliferation in the meristem and developing leaves. To bring further insights into the function of the miR396 regulatory network we performed a mutant enhancer screen of a stable Arabidopsis transgenic line expressing 35S:miR396b, which has a reduction in leaf size. From this screen we recovered several mutants enhancing this phenotype and displaying organs with lotus- or needle-like shape. Analysis of these plants revealed mutations in as2 and rdr6. While 35S:miR396b in an as2 context generated organs with lotus-like shape, the overexpression of the miRNA in an rdr6 mutant background caused more important developmental defects, including pin-like organs and lobed leaves. Combination of miR396 overexpressors, and rdr6 and as2 mutants show additional organ defects, suggesting that the three pathways act in concert. Genetic interactions during leaf development were observed in a similar way between miR396 overexpression and mutants in RDR6, SGS3 or AGO7, which are known to participate in trans-acting siRNA (ta-siRNA) biogenesis. Furthermore, we found that miR396 can cause lotus- and pin-like organs per se, once a certain expression threshold is overcome. In good agreement, mutants accumulating high levels of TCP4, which induces miR396, interacted with the AS1/AS2 pathway to generate lotus-like organs. The results indicate that the miR396 regulatory network and the ta-siRNA biogenesis pathway synergistically interact during leaf development and morphogenesis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Microrna
dc.subject
Mir396
dc.subject
Rdr6
dc.subject
Ta-Sirna
dc.subject
Grf
dc.subject
Tcp
dc.subject.classification
Biología del Desarrollo
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
MicroRNA miR396 and RDR6 synergistically regulate leaf development
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
113
dc.journal.number
1
dc.journal.pagination
2-13
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Mecchia, Martin Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Debernardi, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Rodriguez Virasoro, Ramiro Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Schommer, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.journal.title
Mechanisms of Development
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925477312000810
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mod.2012.07.005
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.mod.2012.07.005
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/22889666
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