Show simple item record

dc.contributor.author Fratebianchi de la Parra, Dante
dc.contributor.author Cavello, Ivana Alejandra
dc.contributor.author Cavalitto, Sebastian Fernando
dc.date.available 2018-06-14T14:10:51Z
dc.date.issued 2017-05
dc.identifier.citation Fratebianchi de la Parra, Dante; Cavello, Ivana Alejandra; Cavalitto, Sebastian Fernando; Purification and Biochemical and Kinetic Properties of an Endo-Polygalacturonase from the Industrial Fungus Aspergillus sojae; Karger; Journal of Molecular Microbiology and Biotechnology; 27; 2; 5-2017; 102-109
dc.identifier.issn 1464-1801
dc.identifier.uri http://hdl.handle.net/11336/48617
dc.description.abstract An endo-polygalacturonase secreted by Aspergillus sojae was characterized after being purified to homogeneity from submerged cultures with orange peel as the sole carbon source by gel filtration and ion-exchange chromatographies. According to SDS-PAGE and analytical isoelectric focusing analyses, the enzyme presents a molecular weight of 47 kDa and pI value of 4.2. This enzyme exhibits considerable stability under highly acidic to neutral conditions (pH 1.5-6.5) and presents a half-life of 2 h at 50°C. Besides its activity towards pectin and polygalacturonic acid, the enzyme displays pectin-releasing activity, acting best in a pH range of 3.3-5.0. Thin-layer chromatographic analysis revealed that tri-galacturonate is the main enzymatic end product of polygalacturonic acid hydrolysis, indicating that it is an endo-polygalacturonase. The enzyme exhibits Michaelis-Menten kinetics, with KM and VMAX values of 0.134 mg/mL and 9.6 μmol/mg/min, respectively, and remained stable and active in the presence of SO2, ethanol, and various cations assayed except Hg2+.
dc.format application/pdf
dc.language.iso eng
dc.publisher Karger
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ASPERGILLUS SOJAE
dc.subject BIOCHEMICAL CHARACTERIZATION
dc.subject ENDO-POLYGALACTURONASE
dc.subject ENZYME PURIFICATION
dc.subject KINETIC PROPERTIES
dc.subject.classification Biotecnología Industrial
dc.subject.classification Biotecnología Industrial
dc.subject.classification INGENIERÍAS Y TECNOLOGÍAS
dc.title Purification and Biochemical and Kinetic Properties of an Endo-Polygalacturonase from the Industrial Fungus Aspergillus sojae
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-06-13T14:18:36Z
dc.journal.volume 27
dc.journal.number 2
dc.journal.pagination 102-109
dc.journal.pais Suiza
dc.description.fil Fil: Fratebianchi de la Parra, Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.description.fil Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.description.fil Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.journal.title Journal of Molecular Microbiology and Biotechnology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1159/000460296
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/460296
dc.conicet.fuente Crossref


Archivos asociados

Icon
Blocked Acceso no disponible

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)