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dc.contributor.author
Pérez Millán, María Inés
dc.contributor.author
Brinkmeier, Michelle
dc.contributor.author
Mortensen, Amanda H
dc.contributor.author
Camper, Sally
dc.date.available
2018-06-13T18:39:40Z
dc.date.issued
2016-06
dc.identifier.citation
Pérez Millán, María Inés; Brinkmeier, Michelle; Mortensen, Amanda H; Camper, Sally; PROP1 triggers epithelial-mesenchymal transition-like process in pituitary stem cells; eLife Sciences Publications Ltd; eLIFE; 5; 6-2016
dc.identifier.issn
2050-084X
dc.identifier.uri
http://hdl.handle.net/11336/48575
dc.description.abstract
Mutations in PROP1 are the most common cause of hypopituitarism in humans; therefore, unraveling its mechanism of action is highly relevant from a therapeutic perspective. Our current understanding of the role of PROP1 in the pituitary gland is limited to the repression and activation of the pituitary transcription factor genes Hesx1 and Pou1f1, respectively. To elucidate the comprehensive PROP1-dependent gene regulatory network, we conducted genome wide analysis of PROP1 DNA binding and effects on gene expression in mutant mice, mouse isolated stem cells and engineered mouse cell lines. We determined that PROP1 is essential for stimulating stem cells to undergo an epithelial to mesenchymal transition-like process necessary for cell migration and differentiation. Genomic profiling reveals that PROP1 binds to genes expressed in epithelial cells like Claudin 23, and to EMT inducer genes like Zeb2, Notch2 and Gli2. Zeb2 activation appears to be a key step in the EMT process. Our findings identify PROP1 as a central transcriptional component of pituitary stem cell differentiation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
eLife Sciences Publications Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Prop1
dc.subject
Stem Cells
dc.subject
Emt
dc.subject
Hipopituitarism
dc.subject.classification
Inmunología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
PROP1 triggers epithelial-mesenchymal transition-like process in pituitary stem cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-06T13:37:43Z
dc.journal.number
5
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Pérez Millán, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.description.fil
Fil: Brinkmeier, Michelle. University of Michigan; Estados Unidos
dc.description.fil
Fil: Mortensen, Amanda H. University of Michigan; Estados Unidos
dc.description.fil
Fil: Camper, Sally. University of Michigan; Estados Unidos
dc.journal.title
eLIFE
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.7554/eLife.14470
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940164/
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