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dc.contributor.author
Scarpeci, Telma Eleonora  
dc.contributor.author
Zanor, María Inés  
dc.contributor.author
Müller Röber, Bernd  
dc.contributor.author
Valle, Estela Marta  
dc.date.available
2016-03-18T18:14:54Z  
dc.date.issued
2013-10  
dc.identifier.citation
Scarpeci, Telma Eleonora; Zanor, María Inés; Müller Röber, Bernd; Valle, Estela Marta; Overexpression of AtWRKY30 enhances abiotic stress tolerance during early growth stages in Arabidopsis thaliana; Springer; Plant Molecular Biology; 83; 3; 10-2013; 265-277  
dc.identifier.issn
0167-4412  
dc.identifier.uri
http://hdl.handle.net/11336/4855  
dc.description.abstract
AtWRKY30 belongs to a higher plant transcription factor superfamily, which responds to pathogen attack. In previous studies, the AtWRKY30 gene was found to be highly and rapidly induced in Arabidopsis thaliana leaves after oxidative stress treatment. In this study, electrophoretic mobility shift assays showed that AtWRKY30 binds with high specificity and affinity to the WRKY consensus sequence (W-box), and also to its own promoter. Analysis of the AtWRKY30 expression pattern by qPCR and using transgenic Arabidopsis lines carrying AtWRKY30 promoter-β-glucuronidase fusions showed transcriptional activity in leaves subjected to biotic or abiotic stress. Transgenic Arabidopsis plants constitutively overexpressing AtWRKY30 (35S::W30 lines) were more tolerant than wild-type plants to oxidative and salinity stresses during seed germination. The results presented here show that AtWRKY30 is responsive to several stress conditions either from abiotic or biotic origin, suggesting that AtWRKY30 could have a role in the activation of defence responses at early stages of Arabidopsis growth by binding to W-boxes found in promoters of many stress/developmentally regulated genes.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Antioxidant Response  
dc.subject
Chloroplast  
dc.subject
Oxidative Stress  
dc.subject
Stress Signaling  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Overexpression of AtWRKY30 enhances abiotic stress tolerance during early growth stages in Arabidopsis thaliana  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2016-03-30 10:35:44.97925-03  
dc.journal.volume
83  
dc.journal.number
3  
dc.journal.pagination
265-277  
dc.journal.pais
Alemania  
dc.journal.ciudad
Berlin  
dc.description.fil
Fil: Scarpeci, Telma Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina  
dc.description.fil
Fil: Zanor, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina  
dc.description.fil
Fil: Müller Röber, Bernd. University of Potsdam. Institute of Biochemistry and Biology; Alemania. Max-Planck Institute of Molecular Plant Physiology; Alemania  
dc.description.fil
Fil: Valle, Estela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina  
dc.journal.title
Plant Molecular Biology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs11103-013-0090-8  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11103-013-0090-8  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/pmid/23794142