Show simple item record

dc.contributor.author Huarte Bonnet, Carla
dc.contributor.author Kumar, Suresh
dc.contributor.author Saparrat, Mario Carlos Nazareno
dc.contributor.author Girotti, Juan Roberto
dc.contributor.author Santana, Marianela
dc.contributor.author Hallsworth, John E.
dc.contributor.author Pedrini, Nicolás
dc.date.available 2018-06-13T17:48:11Z
dc.date.issued 2017-09
dc.identifier.citation Huarte Bonnet, Carla; Kumar, Suresh; Saparrat, Mario Carlos Nazareno; Girotti, Juan Roberto; Santana, Marianela; et al.; Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi: Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes; Humana Press; Applied Biochemistry And Biotechnology; 184; 3; 9-2017; 1047-1060
dc.identifier.issn 0273-2289
dc.identifier.uri http://hdl.handle.net/11336/48547
dc.description.abstract Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. Here, we perform a comparative analysis of CYP genes belonging to clans CYP52 and CYP53 in Aspergillus niger, Beauveria bassiana, Metarhizium robertsii (formerly M. anisopliae var. anisopliae), and Penicillium chrysogenum. These species were able to assimilate n-hexadecane, n-octacosane, and phenanthrene, exhibiting a species-dependent modification in pH of the nutrient medium during this process. Modeling of the molecular docking of the hydrocarbons to the cytochrome P450 active site revealed that both phenanthrene and n-octacosane are energetically favored as substrates for the enzymes codified by genes belonging to both CYP52 and CYP53 clans, and thus appear to be involved in this oxidation step. Analyses of gene expression revealed that CYP53 members were significantly induced by phenanthrene in all species studied, but only CYP52X1 and CYP53A11 from B. bassiana were highly induced with n-alkanes. These findings suggest that the set of P450 enzymes involved in hydrocarbon assimilation by fungi is dependent on phylogeny and reveal distinct substrate and expression specificities.
dc.format application/pdf
dc.language.iso eng
dc.publisher Humana Press
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ASPERGILLUS NIGER
dc.subject BEAUVERIA BASSIANA
dc.subject ENTOMOPATHOGENIC FUNGI
dc.subject HYDROCARBON DEGRADATION
dc.subject METARHIZIUM ANISOPLIAE
dc.subject PENICILLIUM CHRYSOGENUM
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi: Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-06-13T14:20:18Z
dc.journal.volume 184
dc.journal.number 3
dc.journal.pagination 1047-1060
dc.journal.pais Estados Unidos
dc.journal.ciudad Oregon
dc.description.fil Fil: Huarte Bonnet, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil Fil: Kumar, Suresh. University Drive. Department of Diagnostic and Allied Health Sciences; Malasia
dc.description.fil Fil: Saparrat, Mario Carlos Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
dc.description.fil Fil: Girotti, Juan Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil Fil: Santana, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil Fil: Hallsworth, John E.. The Queens University of Belfast; Irlanda
dc.description.fil Fil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.journal.title Applied Biochemistry And Biotechnology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s12010-017-2608-z
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s12010-017-2608-z
dc.conicet.fuente Crossref


Archivos asociados

Icon
Blocked Acceso no disponible

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)