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dc.contributor.author
Huarte Bonnet, Carla  
dc.contributor.author
Kumar, Suresh  
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Saparrat, Mario Carlos Nazareno  
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Girotti, Juan Roberto  
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Santana, Marianela  
dc.contributor.author
Hallsworth, John E.  
dc.contributor.author
Pedrini, Nicolás  
dc.date.available
2018-06-13T17:48:11Z  
dc.date.issued
2017-09  
dc.identifier.citation
Huarte Bonnet, Carla; Kumar, Suresh; Saparrat, Mario Carlos Nazareno; Girotti, Juan Roberto; Santana, Marianela; et al.; Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi: Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes; Humana Press; Applied Biochemistry And Biotechnology; 184; 3; 9-2017; 1047-1060  
dc.identifier.issn
0273-2289  
dc.identifier.uri
http://hdl.handle.net/11336/48547  
dc.description.abstract
Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons. Here, we perform a comparative analysis of CYP genes belonging to clans CYP52 and CYP53 in Aspergillus niger, Beauveria bassiana, Metarhizium robertsii (formerly M. anisopliae var. anisopliae), and Penicillium chrysogenum. These species were able to assimilate n-hexadecane, n-octacosane, and phenanthrene, exhibiting a species-dependent modification in pH of the nutrient medium during this process. Modeling of the molecular docking of the hydrocarbons to the cytochrome P450 active site revealed that both phenanthrene and n-octacosane are energetically favored as substrates for the enzymes codified by genes belonging to both CYP52 and CYP53 clans, and thus appear to be involved in this oxidation step. Analyses of gene expression revealed that CYP53 members were significantly induced by phenanthrene in all species studied, but only CYP52X1 and CYP53A11 from B. bassiana were highly induced with n-alkanes. These findings suggest that the set of P450 enzymes involved in hydrocarbon assimilation by fungi is dependent on phylogeny and reveal distinct substrate and expression specificities.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Humana Press  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Aspergillus Niger  
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Beauveria Bassiana  
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Entomopathogenic Fungi  
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Hydrocarbon Degradation  
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Metarhizium Anisopliae  
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Penicillium Chrysogenum  
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Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Insights into Hydrocarbon Assimilation by Eurotialean and Hypocrealean Fungi: Roles for CYP52 and CYP53 Clans of Cytochrome P450 Genes  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-06-13T14:20:18Z  
dc.journal.volume
184  
dc.journal.number
3  
dc.journal.pagination
1047-1060  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Oregon  
dc.description.fil
Fil: Huarte Bonnet, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
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Fil: Kumar, Suresh. University Drive. Department of Diagnostic and Allied Health Sciences; Malasia  
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Fil: Saparrat, Mario Carlos Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina  
dc.description.fil
Fil: Girotti, Juan Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
dc.description.fil
Fil: Santana, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
dc.description.fil
Fil: Hallsworth, John E.. The Queens University of Belfast; Irlanda  
dc.description.fil
Fil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina  
dc.journal.title
Applied Biochemistry And Biotechnology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s12010-017-2608-z  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s12010-017-2608-z