Artículo
Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis
Garcia Fabiani, Maria Belen
; Montanaro, Mauro Aldo
; Stringa, Pablo Luis
; Lacunza, Ezequiel
; Cattaneo, Elizabeth Renee
; Santana, Marianela
; Pellon Maison, Magali
; Gonzalez Baro, Maria del Rosario
Fecha de publicación:
09/2017
Editorial:
Portland Press
Revista:
Biochemical Journal
ISSN:
0264-6021
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first and rate-limiting step in the de novo glycerolipid synthesis. The GPAT2 isoform differs from the other isoforms because its expression is restricted to male germ cells and cancer cells. It has been recently reported that GPAT2 expression in mouse testis fluctuates during sexual maturation and that it is regulated by epigenetic mechanisms in combination with vitamin A derivatives. Despite progress made in this field, information about GPAT2 role in the developing male germ cells remains unclear. The aim of the present study was to confirm the hypothesis that GPAT2 is required for the normal physiology of testes and male germ cell maturation. The gene was silenced in vivo by inoculating lentiviral particles carrying the sequence of a short-hairpin RNA targeting Gpat2 mRNA into mouse testis. Histological and gene expression analysis showed impaired spermatogenesis and arrest at the pachytene stage. Defects in reproductive fitness were also observed, and the analysis of apoptosis-related gene expression demonstrated the activation of apoptosis in Gpat2-silenced germ cells. These findings indicate that GPAT2 protein is necessary for the normal development of male gonocytes, and that its absence triggers apoptotic mechanisms, thereby decreasing the number of dividing germ cells.
Palabras clave:
Meiosis
,
Apoptosis
,
Mouse
,
Gene Silencing
,
Germ Cell
,
Gpat2
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(INIBIOLP)
Articulos de INST.DE INVEST.BIOQUIMICAS DE LA PLATA
Articulos de INST.DE INVEST.BIOQUIMICAS DE LA PLATA
Citación
Garcia Fabiani, Maria Belen; Montanaro, Mauro Aldo; Stringa, Pablo Luis; Lacunza, Ezequiel; Cattaneo, Elizabeth Renee; et al.; Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis; Portland Press; Biochemical Journal; 474; 18; 9-2017; 3093-3107
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