Mostrar el registro sencillo del ítem

dc.contributor.author
Rivadeneira, Josefina  
dc.contributor.author
Di Virgilio, Ana Laura  
dc.contributor.author
Audisio, Marcela Carina  
dc.contributor.author
Boccaccini, Aldo R.  
dc.contributor.author
Gorustovich Alonso, Alejandro Adrian  
dc.date.available
2018-06-12T18:48:00Z  
dc.date.issued
2016-11  
dc.identifier.citation
Rivadeneira, Josefina; Di Virgilio, Ana Laura; Audisio, Marcela Carina; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian; 45S5 Bioglass® concentrations modulate the release of vancomycin hydrochloride from gelatin–starch films: evaluation of antibacterial and cytotoxic effects; Springer; Journal of Materials Science; 52; 15; 11-2016; 9091-9102  
dc.identifier.issn
0022-2461  
dc.identifier.uri
http://hdl.handle.net/11336/48397  
dc.description.abstract
The aim of this work was to evaluate the release profile of vancomycin hydrochloride (VC), as well as the degradation, in vitro antistaphylococcal effect and cytotoxicity in MG-63 osteoblast-like cells of gelatin–starch (GS) films added with different concentrations of microparticles of the bioactive glass 45S5 (m-BG). The biomaterials were obtained through the gel-casting method. Four different composites were prepared at four different weight percentages of m-BG: 0, 5, 10, and 15 %. Glutaraldehyde 0.25 wt% (GA) was used as the cross-linker. The composites were characterized by scanning electron microscopy and the in vitro degradation of the films was studied by measuring the water uptake and weight loss. The drug release kinetics was quantified spectrophotometrically. The inhibition zone test and the plate count method were used to evaluate the antibacterial activity of the samples. Three staphylococcus strains were evaluated: Staphylococcus aureus ATCC6538, S. aureus ATCC29213, and Staphylococcus epidermidis ATCC12228. Cytotoxicity effects were evaluated through the MTT assay. The addition of m-BG to GS films showed no effects on the amount of water uptake, but led to an increase in the weight loss over time, even with m-BG content. The release rate of VC was also affected by the increasing concentration of m-BG in the composite films. However, the antibacterial effects of the composites were not improved by this modulation. All composites strongly inhibited staphylococcal cells with similar strength. On the other hand, liquid extracts from the composites resulted in cytotoxic effects on MG-63 osteoblast-like cells due to the presence of GA, but not to the concentration of VC or m-BG.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Vancomycin Hydrochloride  
dc.subject
Antibacterial  
dc.subject
Cytotoxicity  
dc.subject
Gelatin-Starch  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
45S5 Bioglass® concentrations modulate the release of vancomycin hydrochloride from gelatin–starch films: evaluation of antibacterial and cytotoxic effects  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-06-12T13:30:42Z  
dc.identifier.eissn
1573-4803  
dc.journal.volume
52  
dc.journal.number
15  
dc.journal.pagination
9091-9102  
dc.journal.pais
Alemania  
dc.description.fil
Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina. Universidad Católica de Salta; Argentina  
dc.description.fil
Fil: Di Virgilio, Ana Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina  
dc.description.fil
Fil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; Alemania  
dc.description.fil
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina. Universidad Católica de Salta; Argentina  
dc.journal.title
Journal of Materials Science  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s10853-016-0512-5  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10853-016-0512-5