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dc.contributor.author
Wald, Flavia A.
dc.contributor.author
Kissen, Ralph
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du Jardin, Patrick
dc.contributor.author
Moreno, Silvia
dc.date.available
2018-06-12T18:32:40Z
dc.date.issued
2003-02
dc.identifier.citation
Wald, Flavia A.; Kissen, Ralph; du Jardin, Patrick; Moreno, Silvia; Characterization of UDP-glucose: protein transglucosylase genes from potato.; Springer; Plant Molecular Biology; 52; 4; 2-2003; 705-714
dc.identifier.issn
0167-4412
dc.identifier.uri
http://hdl.handle.net/11336/48390
dc.description.abstract
Many plant autocatalytic glycosyltransferases are implicated in plant polysaccharide biosynthesis. Cloning of cDNAs encoding potato (Solanum tuberosum L.) UDP-Glc:protein transglucosylase (UPTG, EC 2.4.1.112) and expression of the cDNA clone E11 in Escherichia coli have been previously reported. Here, we studied the functional expression of a second cDNA of the enzyme (E2 clone). Northern blots analysis, with specific cDNA probes for the two UPTG isoforms, showed a differential expression pattern of mRNA levels in different potato tissues. Moreover, both UPTG recombinant enzymes showed different kinetic parameters. The recombinant protein encoded by E2 clone has an apparent Imax for UDP-Xyl and UDP-Gal, significantly higher than for UDP-Glc. The Km values for UDP-Glc were 0.45-0.71 microM and the values for UDP-Xyl and UDP-Gal were slightly higher than that of the UDP-Glc (1.2-2.71 microM) for both UPTG recombinant enzymes. The present study revealed further evidence for the proposed role of UPTG in the synthesis of cell wall polysaccharide. It was found a correlation between UPTG transcript levels and the growing state of the tissues in which there was an active synthesis of cell wall components. Southern blot analysis indicates that at least three genes encoding UPTG are present in potato genome. Phylogenetic analysis of both UPTG recombinant proteins showed that they are members of the RGP subfamilies from dicots.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Autocatalytic Glycosyltransferase
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Cell-Wall
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Polysaccharide Synthesis
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Udp-Glucose
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Characterization of UDP-glucose: protein transglucosylase genes from potato.
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-05-10T15:46:13Z
dc.identifier.eissn
1573-5028
dc.journal.volume
52
dc.journal.number
4
dc.journal.pagination
705-714
dc.journal.pais
Alemania
dc.journal.ciudad
Dordrecht
dc.description.fil
Fil: Wald, Flavia A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Kissen, Ralph. Gembloux Agricultural University; Bélgica
dc.description.fil
Fil: du Jardin, Patrick. Gembloux Agricultural University; Bélgica
dc.description.fil
Fil: Moreno, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.journal.title
Plant Molecular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1023/A:1025061324856
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1023/A:1025061324856
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