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dc.contributor.author
Parisi, Monica  
dc.contributor.author
Moreno, Silvia  
dc.contributor.author
Fernández, Graciela  
dc.date.available
2018-06-12T18:22:43Z  
dc.date.issued
2002-11  
dc.identifier.citation
Parisi, Monica; Moreno, Silvia; Fernández, Graciela; Characterization of a novel peptidase from tissue culture of garlic (Allium sativum.L); Springer; In Vitro Cellular ; 38; 6; 11-2002; 608-612  
dc.identifier.issn
1054-5476  
dc.identifier.uri
http://hdl.handle.net/11336/48383  
dc.description.abstract
A simple and efficient medium for callus tissue culture from garlic to obtain maximal proteolytic activity is described. Murashige and Skoog basal medium supplemented with 4.44 μM naphthaleneacetic acid (NAA) and 0.54 μM benzyladenine (BA) resulted in the best biomass production and protease expression. The protease activity belongs to the class of cysteine proteases since they are inhibited by E64 and Leupeptin and also they are activated by 2-mercaptoethanol and cysteine. They showed good thermal stability. Three active protease bands were found in zymograms of Allium sativum. The in vitro system revealed a significantly higher protease level than storage and embryo tissues of in vivo bulbs.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Callus Culture  
dc.subject
Cysteine Protease  
dc.subject
Endopeptidase  
dc.subject
In Vitro Culture  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Characterization of a novel peptidase from tissue culture of garlic (Allium sativum.L)  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-05-10T15:46:11Z  
dc.journal.volume
38  
dc.journal.number
6  
dc.journal.pagination
608-612  
dc.journal.pais
Alemania  
dc.journal.ciudad
IOwa  
dc.description.fil
Fil: Parisi, Monica. Universidad Nacional de Luján; Argentina  
dc.description.fil
Fil: Moreno, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina  
dc.description.fil
Fil: Fernández, Graciela. Universidad Nacional de Luján; Argentina  
dc.journal.title
In Vitro Cellular  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1079/IVP2002344  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1079/IVP2002344  

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