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dc.contributor.author
Anaya, G.
dc.contributor.author
Molina, A.
dc.contributor.author
Valera, Maria Analia
dc.contributor.author
Moreno Millán, Miguel
dc.contributor.author
Azor, P.
dc.contributor.author
Peral Garcia, Pilar
dc.contributor.author
Demyda-Peyrás, Sebastian
dc.date.available
2018-06-11T17:33:08Z
dc.date.issued
2017-08
dc.identifier.citation
Anaya, G.; Molina, A.; Valera, Maria Analia; Moreno Millán, Miguel; Azor, P.; et al.; Sex chromosomal abnormalities associated with equine infertility: validation of a simple molecular screening tool in the Purebred Spanish Horse; Wiley Blackwell Publishing, Inc; Animal Genetics; 48; 4; 8-2017; 412-419
dc.identifier.issn
0268-9146
dc.identifier.uri
http://hdl.handle.net/11336/48111
dc.description.abstract
Chromosomal abnormalities in the sex chromosome pair (ECAX and ECAY) are widely associated with reproductive problems in horses. However, a large proportion of these abnormalities remains undiagnosed due to the lack of an affordable diagnostic tool that allows for avoiding karyotyping tests. Hereby, we developed an STR (single-tandem-repeat)-based molecular method to determine the presence of the main sex chromosomal abnormalities in horses in a fast, cheap and reliable way. The frequency of five ECAX-linked (LEX026, LEX003, TKY38, TKY270 and UCDEQ502) and two ECAY-linked (EcaYH12 and SRY) markers was characterized in 261 Purebred Spanish Horses to determine the efficiency of the methodology developed to be used as a chromosomal diagnostic tool. All the microsatellites analyzed were highly polymorphic, with a sizeable number of alleles (polymorphic information content > 0.5). Based on this variability, the methodology showed 100% sensitivity and 99.82% specificity to detect the most important sex chromosomal abnormalities reported in horses (chimerism, Turner's syndrome and sex reversal syndromes). The method was also validated with 100% efficiency in 10 individuals previously diagnosed as chromosomally aberrant. This STR screening panel is an efficient and reliable molecular–cytogenetic tool for the early detection of sex chromosomal abnormalities in equines that could be included in breeding programs to save money, effort and time of veterinary practitioners and breeders.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CHIMERISM
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DIAGNOSTIC TOOL
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EQUINES
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GENETIC INFERTILITY
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MICROSATELLITES
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MOLECULAR CHARACTERIZATION
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MOSAICISM
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SEX REVERSAL SYNDROME
dc.subject
TURNER SYNDROME
dc.subject.classification
Otras Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Sex chromosomal abnormalities associated with equine infertility: validation of a simple molecular screening tool in the Purebred Spanish Horse
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-11T14:27:40Z
dc.journal.volume
48
dc.journal.number
4
dc.journal.pagination
412-419
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Anaya, G.. Universidad de Córdoba; España
dc.description.fil
Fil: Molina, A.. Universidad de Córdoba; España
dc.description.fil
Fil: Valera, Maria Analia. Universidad de Sevilla; España
dc.description.fil
Fil: Moreno Millán, Miguel. Universidad de Sevilla; España
dc.description.fil
Fil: Azor, P.. Universidad de Sevilla; España
dc.description.fil
Fil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.description.fil
Fil: Demyda-Peyrás, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
dc.journal.title
Animal Genetics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/age.12543
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/age.12543
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