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dc.contributor.author
Dorado, J.  
dc.contributor.author
Gálvez, M.J.  
dc.contributor.author
Demyda-peyrás, Sebastian  
dc.contributor.author
Ortiz, I.  
dc.contributor.author
Morrell, J.M.  
dc.contributor.author
Crespo, F.  
dc.contributor.author
Gósalvez, J.  
dc.contributor.author
Hidalgo, M.  
dc.date.available
2018-06-11T17:31:22Z  
dc.date.issued
2016-04  
dc.identifier.citation
Dorado, J.; Gálvez, M.J.; Demyda-peyrás, Sebastian; Ortiz, I.; Morrell, J.M.; et al.; Differences in preservation of canine chilled semen using simple sperm washing, single-layer centrifugation and modified swim-up preparation techniques; Csiro Publishing; Reproduction Fertility and Development; 28; 10; 4-2016; 1545-1552  
dc.identifier.issn
1031-3613  
dc.identifier.uri
http://hdl.handle.net/11336/48093  
dc.description.abstract
This study compared the efficacy of simple sperm washing (SW), single-layer centrifugation (SLC) and modified swim-up (SU) techniques in the preparation of dog spermatozoa for cooling. Eighteen ejaculates, collected from three dogs (six per dog), were pooled (three ejaculates per pool) and divided into three aliquots: (1) one aliquot was washed and cooled at 5°C for 72h, considered as control (SW-control), (2) the second aliquot was selected by SLC through Androcoll-C and subsequently cooled in the same way as the SW-control samples (SLC-AC) and (3) the last aliquot was selected by a modified SU method with Androcoll-C and cooled as mentioned above (SU-AC). Assessment of sperm motility, sperm morphology, sperm membrane integrity and acrosome integrity were performed on aliquots of fresh semen and chilled-rewarmed samples. Sperm membrane integrity and progressive motility were significantly (P<0.05) improved by SU-AC compared with SW-control. Morphological sperm abnormalities decreased significantly (P<0.001) in SLC-AC samples compared with SW-control samples. These sperm variables did not differ between SLC-AC and SU-AC methods (P>0.05). The recovery rates were not significantly (P>0.05) different between SW-control, SLC-AC and SU-AC samples. Our results confirm that SU-AC may be a successful method for the preparation of dog spermatozoa for cooling.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Csiro Publishing  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Androcoll-C  
dc.subject
Chilled Dog Semen  
dc.subject
Colloid  
dc.subject
Sperm Migration  
dc.subject
Sperm Selection.  
dc.subject.classification
Otras Biotecnología Agropecuaria  
dc.subject.classification
Biotecnología Agropecuaria  
dc.subject.classification
CIENCIAS AGRÍCOLAS  
dc.title
Differences in preservation of canine chilled semen using simple sperm washing, single-layer centrifugation and modified swim-up preparation techniques  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-06-11T14:31:36Z  
dc.journal.volume
28  
dc.journal.number
10  
dc.journal.pagination
1545-1552  
dc.journal.pais
Australia  
dc.journal.ciudad
Collingwood  
dc.description.fil
Fil: Dorado, J.. Universidad de Córdoba; España  
dc.description.fil
Fil: Gálvez, M.J.. Universidad de Córdoba; España  
dc.description.fil
Fil: Demyda-peyrás, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad de Córdoba; España  
dc.description.fil
Fil: Ortiz, I.. Universidad de Córdoba; España  
dc.description.fil
Fil: Morrell, J.M.. Swedish: Sveriges Lantbruksuniversitet; Suecia  
dc.description.fil
Fil: Crespo, F.. Ministerio de Defensa. Fondo de Explotación de los Servicios de Cría Caballar y Remonta. Centro Militar de Veterinaria de la Defensa; España  
dc.description.fil
Fil: Gósalvez, J.. Universidad Autónoma de Madrid; España  
dc.description.fil
Fil: Hidalgo, M.. Universidad de Córdoba; España  
dc.journal.title
Reproduction Fertility and Development  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1071/RD15071  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/rd/RD15071