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dc.contributor.author de Francesco, Agustina
dc.contributor.author Costa, N.
dc.contributor.author Garcia, Maria Laura
dc.date.available 2018-06-08T18:52:02Z
dc.date.issued 2017-04
dc.identifier.citation de Francesco, Agustina; Costa, N.; Garcia, Maria Laura; Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes; Springer; Transgenic Research; 26; 2; 4-2017; 225-235
dc.identifier.issn 0962-8819
dc.identifier.uri http://hdl.handle.net/11336/47933
dc.description.abstract Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation. In particular, line ihpCP-15 has been resistant for more than 2 years, even after re-inoculation. The ihpCP plants were also resistant against a heterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP-15 manifested complete resistance while line ihpCP-10 was tolerant to the virus, although with variable behaviour, showing delay and attenuation in PsB symptoms. These lines are promising for a biotech product aimed at eradicating psorosis.
dc.format application/pdf
dc.language.iso eng
dc.publisher Springer
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CITRUS PSOROSIS VIRUS
dc.subject HAIRPIN-RNA
dc.subject PSOROSIS A
dc.subject PSOROSIS B
dc.subject TRANSGENIC SWEET ORANGE
dc.subject VIRUS RESISTANCE
dc.subject.classification Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental
dc.subject.classification Biotecnología del Medio Ambiente
dc.subject.classification INGENIERÍAS Y TECNOLOGÍAS
dc.title Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-06-08T14:30:14Z
dc.journal.volume 26
dc.journal.number 2
dc.journal.pagination 225-235
dc.journal.pais Países Bajos
dc.journal.ciudad Dordrech
dc.description.fil Fil: de Francesco, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
dc.description.fil Fil: Costa, N.. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
dc.journal.title Transgenic Research
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s11248-016-0001-2
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11248-016-0001-2
dc.conicet.fuente Elsevier


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)