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dc.contributor.author
Blanco Fernandez, Maria Dolores
dc.contributor.author
Barrios, Melina Elizabeth
dc.contributor.author
Cammarata, Robertina Viviana
dc.contributor.author
Torres, Carolina
dc.contributor.author
Taboga, Oscar Alberto
dc.contributor.author
Mbayed, Viviana Andrea
dc.date.available
2018-06-08T18:18:26Z
dc.date.issued
2017-05
dc.identifier.citation
Blanco Fernandez, Maria Dolores; Barrios, Melina Elizabeth; Cammarata, Robertina Viviana; Torres, Carolina; Taboga, Oscar Alberto; et al.; Comparison of internal process control viruses for detection of food and waterborne viruses; Springer; Applied Microbiology and Biotechnology; 101; 10; 5-2017; 4289-4298
dc.identifier.issn
0175-7598
dc.identifier.uri
http://hdl.handle.net/11336/47917
dc.description.abstract
Enteric viruses are pathogens associated with food- and waterborne outbreaks. The recovery of viruses from food or water samples is affected by the procedures applied to detect and concentrate them. The incorporation of an internal process control virus to the analyses allows monitoring the performance of the methodology. The aim of this study was to produce a recombinant adenovirus (rAdV) and apply it together with bacteriophage PP7 as process controls. The rAdV carries a DNA construction in its genome to differentiate it from wild-type adenovirus by qPCR. The stability of both control viruses was evaluated at different pH conditions. The rAdV was stable at pH 3, 7, and 10 for 18 h. PP7 infectious particles were stable at pH 7 and showed a 2.14 log reduction at pH 10 and total decay at pH 3 after 18 h. Three virus concentration methods were evaluated: hollow-fiber tap water ultrafiltration, wastewater ultracentrifugation, and elution-PEG precipitation from lettuce. Total and infectious viruses were quantified and their recoveries were calculated. Virus recovery for rAdV and PP7 by ultrafiltration showed a wide range (2.10?84.42 and 13.54?84.62%, respectively), whereas that by ultracentrifugation was 5.05?13.71 and 6.98?13.27%, respectively. The performance of ultracentrifugation to concentrate norovirus and enteroviruses present in sewage was not significantly different to the recovery of control viruses. For detection of viruses from lettuce, genomic copies of PP7 were significantly more highly recovered than adenovirus (14.74?18.82 and 0.00?3.44%, respectively). The recovery of infectious virus particles was significantly affected during sewage ultracentrifugation and concentration from lettuce. The simultaneous use of virus controls with dissimilar characteristics and behaviors might resemble different enteric viruses.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hollow Fiber Ultrafiltration
dc.subject
Internal Process Control Virus
dc.subject
Lettuce
dc.subject
Sewage
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Ultracentrifugation
dc.subject
Virus Recovery
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Comparison of internal process control viruses for detection of food and waterborne viruses
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-07T14:28:21Z
dc.journal.volume
101
dc.journal.number
10
dc.journal.pagination
4289-4298
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Blanco Fernandez, Maria Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
dc.description.fil
Fil: Barrios, Melina Elizabeth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Ministerio de Ciencia y Tecnología. Agencia Nacional de Promoción Científica y Tecnológica; Argentina
dc.description.fil
Fil: Cammarata, Robertina Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
dc.description.fil
Fil: Torres, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
dc.description.fil
Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Mbayed, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
dc.journal.title
Applied Microbiology and Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s00253-017-8244-2
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00253-017-8244-2
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