Artículo
Essential dynamics of the cold denaturation: pressure and temperature effects in yeast frataxin
Fecha de publicación:
11/2016
Editorial:
Wiley-liss, Div John Wiley & Sons Inc
Revista:
Proteins: Structure, Function And Genetics
ISSN:
0887-3585
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The cold denaturation of globular proteins is a process that can be caused by increasing pressure or decreasing the temperature. Currently, the action mechanism of this process has not been clearly understood, raising an interesting debate on the matter. We have studied the process of cold denaturation using molecular dynamics simulations of the frataxin system Yfh1, which has a dynamic experimental characterization of unfolding at low and high temperatures. The frataxin model here studied allows a comparative analysis using experimental data. Furthermore, we monitored the cold denaturation process of frataxin and also investigated the effect under the high‐pressure regime. For a better understanding of the dynamics and structural properties of the cold denaturation, we also analyzed the MD trajectories using essentials dynamic. The results indicate that changes in the structure of water by the effect of pressure and low temperatures destabilize the hydrophobic interaction modifying the solvation and the system volume leading to protein denaturation.
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Articulos(CEQUINOR)
Articulos de CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Articulos de CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Articulos(IFLYSIB)
Articulos de INST.FISICA DE LIQUIDOS Y SIST.BIOLOGICOS (I)
Articulos de INST.FISICA DE LIQUIDOS Y SIST.BIOLOGICOS (I)
Citación
Espinosa Silva, Yanis Ricardo; Grigera, Jose Raul; Caffarena, Ernesto Raúl; Essential dynamics of the cold denaturation: pressure and temperature effects in yeast frataxin; Wiley-liss, Div John Wiley & Sons Inc; Proteins: Structure, Function And Genetics; 85; 1; 11-2016; 125-136
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