Artículo
Chlorpyrifos induces endoplasmic reticulum stress in JEG-3 cells
Reyna, Luciana
; Flores Martín, Jésica Belén
; Ridano, Magali Evelin
; Panzetta-Dutari, Graciela Maria del Valle
; Genti de Raimondi, Susana
Fecha de publicación:
04/2017
Editorial:
Pergamon-Elsevier Science Ltd
Revista:
Toxicology in Vitro
ISSN:
0887-2333
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Chlorpyrifos (CPF) is an organophosphorous pesticide widely used in agricultural, industrial, and household applications. We have previously shown that JEG-3 cells are able to attenuate the oxidative stress induced by CPF through the adaptive activation of the Nrf2/ARE pathway. Considering that there is a relationship between oxidative stress and endoplasmic reticulum stress (ER), herein we investigated whether CPF also induces ER stress in JEG-3 cells. Cells were exposed to 50 μM or 100 μM CPF during 24 h in conditions where cell viability was not altered. Western blot and PCR assays were used to explore the protein and mRNA levels of ER stress biomarkers, respectively. CPF induced an increase of the typical ER stress-related proteins, such as GRP78/BiP and IRE1α, a sensor for the unfolded protein response, as well as in phospho-eIF2α and XBP1 mRNA splicing. Additionally, CPF led to a decrease in p53 protein expression. The downregulation of p53 levels induced by CPF was partially blocked when cells were exposed to CPF in the presence of the proteasome inhibitor MG132. Altogether, these findings point out that CPF induces ER stress in JEG-3 cells; however these cells are able to attenuate it downregulating the levels of the pro-apoptotic protein p53.
Palabras clave:
Chlorpyrifos
,
Endoplasmic Reticulum Stress
,
P53
,
Jeg-3 Cells
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Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Reyna, Luciana; Flores Martín, Jésica Belén; Ridano, Magali Evelin; Panzetta-Dutari, Graciela Maria del Valle; Genti de Raimondi, Susana; Chlorpyrifos induces endoplasmic reticulum stress in JEG-3 cells; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 40; 4-2017; 88-93
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