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Artículo

Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups

Mamone, Leandro ArielIcon ; Ferreyra, Darío DavidIcon ; Gándara, LautaroIcon ; Di Venosa, Gabriela MarianaIcon ; Vallecorsa, Pablo DanielIcon ; Saenz, Daniel AlbertoIcon ; Calvo, Gustavo HernánIcon ; Batlle, Alcira María del C.Icon ; Buzzola, Fernanda RoxanaIcon ; Durantini, Edgardo NéstorIcon ; Casas, Adriana GabrielaIcon
Fecha de publicación: 08/2016
Editorial: Elsevier Science Sa
Revista: Journal of Photochemistry and Photobiology B: Biology
ISSN: 1011-1344
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Medicina Química

Resumen

Biofilm-associated diseases account for 80% of all infections in humans. Due to the emergence of antibiotic resistances, alternative therapies such as Photodynamic Inactivation (PDI) of microorganisms have emerged. Porphyrins with intrinsic positive charges have been proposed as successful photosensitizers (PSs) against microorganisms. We have recently designed the new synthetic porphyrin 5,10,15,20-tetrakis[4-(3-N,N-dimethylammoniumpropoxy)phenyl]porphyrin (TAPP) containing four basic amine groups in the periphery of the tetrapyrrolic macrocycle, which can acquire positive charges at physiological pH, thus favouring the interaction with biomembranes. Illumination of planktonic cultures of Staphylococcus aureus at 180 J/cm2 in the presence of 2.5 μM TAPP induced complete bacteria eradication. For the TAPP-PDI treatment of S. aureus biofilms, higher light fluences and PS concentrations were needed. Employing 20 μM TAPP and 180 J/cm2, around 3-log CFU reduction were obtained. In order to determine the efficacy of TAPP-PDI on Gram-negative bacteria, we performed planktonic and biofilm assays employing Pseudomonas aeruginosa. Much higher TAPP doses as compared to S. aureus were needed to achieve planktonic bacteria photosensitization (3-log CFU reduction at 20 μM TAPP and 180 J/cm2). On the other hand, high concentrations of TAPP were nontoxic to P. aeruginosa growing on biofilms, and employing 30 μM TAPP and 180 J/cm2 we obtained 3-log CFU reduction. The main conclusion of the present work is that TAPP is a promising and efficient PS capable of promoting photodynamic killing of both Gram-negative and -positive in planktonic bacteria, though more effectively in the latter. In addition, TAPP-PDI induces similar photoinactivation rates in both bacteria types growing on biofilms, with lower dark toxicity in the Gram-negative one.
Palabras clave: Bacteria , Biofilm , Photodynamic Inactivation , Planktonic , Porphyrin , Staphylococcus Aureus
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/47806
URL: https://www.sciencedirect.com/science/article/pii/S1011134415302396
DOI: http://dx.doi.org/10.1016/j.jphotobiol.2016.05.026
Colecciones
Articulos(INIFTA)
Articulos de INST.DE INV.FISICOQUIMICAS TEORICAS Y APLIC.
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos(CIPYP)
Articulos de CENTRO DE INVEST. SOBRE PORFIRINAS Y PORFIRIAS
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Mamone, Leandro Ariel; Ferreyra, Darío David; Gándara, Lautaro; Di Venosa, Gabriela Mariana; Vallecorsa, Pablo Daniel; et al.; Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups; Elsevier Science Sa; Journal of Photochemistry and Photobiology B: Biology; 161; 8-2016; 222-229
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