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dc.contributor.author Joray, Mariana Belén
dc.contributor.author Villafañez, Florencia
dc.contributor.author González, María Laura
dc.contributor.author Crespo, Maria Ines
dc.contributor.author Laiolo, Jerónimo
dc.contributor.author Palacios, Sara Maria
dc.contributor.author Bocco, Jose Luis
dc.contributor.author Soria, Ramiro Gaston
dc.contributor.author Carpinella, Maria Cecilia
dc.date.available 2018-06-07T20:57:02Z
dc.date.issued 2017-11-29
dc.identifier.citation Joray, Mariana Belén; Villafañez, Florencia; González, María Laura; Crespo, Maria Ines; Laiolo, Jerónimo; et al.; P53 tumor suppressor is required for efficient execution of the death program following treatment with a cytotoxic limonoid obtained from Melia azedarach; Pergamon-Elsevier Science Ltd; Food And Chemical Toxicology; 109; Part 2; 29-11-2017; 888-897
dc.identifier.issn 0278-6915
dc.identifier.uri http://hdl.handle.net/11336/47796
dc.description.abstract This work examines the antitumor activity of an isomeric mixture (1), composed of the limonoids meliartenin and its interchangeable isomer 12-hydroxyamoorastatin. The results obtained showed that 1 displayed outstanding cytotoxic activity against CCRF-CEM, K562, A549 and HCT116 cells, with a highly selective effect on the latter, with an IC50 value of 0.2 μM. Based on this finding, HCT116 cells were selected to study the mechanism of action of 1. Cell cycle analysis revealed that 1 induced sustained arrest in the S-phase, which was followed by the triggering of apoptotic cell death and reduced clonogenic capacity. This cytotoxicity was seen to be preceded by the upregulation of the tumor suppressor p53 and its target effector p21. In addition, it was found that p53 expression was required for efficient cell death induction, and thus that the toxicity of 1 relies mainly on p53-dependent mechanisms. Taken together, these findings position 1 as a potent antitumor agent, with potential for the development of novel chemotherapeutic drugs based on the induction of S-phase arrest.
dc.format application/pdf
dc.language.iso eng
dc.publisher Pergamon-Elsevier Science Ltd
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject MELIARTENIN12
dc.subject HYDROXYAMOORASTATIN
dc.subject CYTOTOXICITY
dc.subject APOPTOSIS
dc.subject CELL CYCLE ARREST
dc.subject HCT116
dc.subject P53
dc.subject.classification Inmunología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title P53 tumor suppressor is required for efficient execution of the death program following treatment with a cytotoxic limonoid obtained from Melia azedarach
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-05-30T17:20:32Z
dc.journal.volume 109
dc.journal.number Part 2
dc.journal.pagination 888-897
dc.journal.pais Países Bajos
dc.journal.ciudad Amsterdam
dc.description.fil Fil: Joray, Mariana Belén. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Villafañez, Florencia. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil Fil: González, María Laura. Universidad Católica de Córdoba; Argentina
dc.description.fil Fil: Crespo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Católica de Córdoba; Argentina
dc.description.fil Fil: Laiolo, Jerónimo. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Palacios, Sara Maria. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Bocco, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil Fil: Soria, Ramiro Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil Fil: Carpinella, Maria Cecilia. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Food And Chemical Toxicology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S027869151730217X
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.fct.2017.04.039
dc.conicet.fuente Elsevier


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  • Articulos(IRNASUS) [6]
    Articulos de INSTITUTO DE INVESTIGACIONES EN RECURSOS NATURALES Y SUSTENTABILIDAD JOSE SANCHEZ LABRADOR S.J.
  • Articulos(CIBICI) [246]
    Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
  • Articulos(CCT - CORDOBA) [2426]
    Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA

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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)