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dc.contributor.author Di Gregorio, Sabrina Noelia
dc.contributor.author Fernandez, Silvina
dc.contributor.author Cuirolo, Arabela Ximena
dc.contributor.author Verlaine, Olivier
dc.contributor.author Amoroso, Ana
dc.contributor.author Mengin Lecreulx, Dominique
dc.contributor.author Famiglietti, Angela María Rosa
dc.contributor.author Joris, Bernard
dc.contributor.author Mollerach, Marta Eugenia
dc.date.available 2018-06-07T18:29:28Z
dc.date.issued 2017-01
dc.identifier.citation Di Gregorio, Sabrina Noelia; Fernandez, Silvina; Cuirolo, Arabela Ximena; Verlaine, Olivier; Amoroso, Ana; et al.; Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain; Mary Ann Liebert; Microbial Drug Resistance: Mechanisms Epidemiology and Disease; 23; 1; 1-2017; 44-50
dc.identifier.issn 1076-6294
dc.identifier.uri http://hdl.handle.net/11336/47723
dc.description.abstract The aim of this study is to characterize the factors related to peptidoglycan metabolism in isogenic hVISA/VISA ST100 strains. Recently, we reported the increase in IS256 transposition in invasive hVISA ST100 clinical strains isolated from the same patient (D1 and D2) before and after vancomycin treatment and two laboratory VISA mutants (D23C9 and D2P11) selected from D2 in independent experiments. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of peptidoglycan muropeptides showed increased proportion of monomeric muropeptides and a concomitant decrease in the proportion of tetrameric muropeptide in D2 and derived mutants when compared to the original strain D1. In addition, strain D2 and its derived mutants showed an increase in cell wall thickness with increased pbp2 gene expression. The VISA phenotype was not stable in D2P11 and showed a reduced autolysis profile. On the other hand, the mutant D23C9 differentiates from D2 and D2P11 in the autolysis profile, and pbp4 transcription profile. D2-derived mutants exhibited differences in the susceptibility to other antimicrobials. Our results highlight the possibility of selection of different VISA phenotypes from a single hVISA-ST100 genetic background.
dc.format application/pdf
dc.language.iso eng
dc.publisher Mary Ann Liebert
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject STAPHYLOCOCCUS AUREUS
dc.subject VANCOMYCIN
dc.subject hVISA
dc.subject VIS
dc.subject MRSA ST100
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-06-07T14:25:04Z
dc.journal.volume 23
dc.journal.number 1
dc.journal.pagination 44-50
dc.journal.pais Estados Unidos
dc.description.fil Fil: Di Gregorio, Sabrina Noelia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Fernandez, Silvina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina
dc.description.fil Fil: Cuirolo, Arabela Ximena. Université de Liège; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Verlaine, Olivier. Université de Liège; Bélgica
dc.description.fil Fil: Amoroso, Ana. Université de Liège; Bélgica
dc.description.fil Fil: Mengin Lecreulx, Dominique. Université Paris Sud; Francia
dc.description.fil Fil: Famiglietti, Angela María Rosa. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
dc.description.fil Fil: Joris, Bernard. Université de Liège; Bélgica
dc.description.fil Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Microbial Drug Resistance: Mechanisms Epidemiology and Disease
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1089/mdr.2016.0160
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/mdr.2016.0160
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)