Artículo
The expansion of adult stem/progenitor cells and their marker expression fluctuations are linked with pituitary plastic adaptation during gestation and lactancy
Vaca, Alicia Maldré del Valle
; Guido, Carolina Beatriz
; Sosa, Liliana del Valle
; Nicola, Juan Pablo
; Mukdsi, Jorge Humberto; Petiti, Juan Pablo
; Torres, AIicia Inés
Fecha de publicación:
08/2016
Editorial:
American Physiological Society
Revista:
American Journal Of Physiology-endocrinology And Metabolism
ISSN:
0193-1849
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Extensive evidence has revealed variations in the number of hormone-producing cells in the pituitary gland, which occur under physiological conditions such as gestation and lactancy. It has been proposed that new hormone-producing cells differentiate from stem cells. However, exactly how and when this takes place is not clear. In this work, we used immunoelectron microscopy to identify adult pituitary stem/progenitor cells (SC/P) localized in the marginal zone (MZ), and additionally, we detected GFRa2-, Sox2-, and Sox9-positive cells in the adenoparenchyma (AP) by fluorescence microscopy. Then, we evaluated fluctuations of SC/P mRNA and protein level markers in MZ and AP during gestation and lactancy. An upregulation in stemness markers was shown at term of gestation (AT) in MZ, whereas there were more progenitor cell markers in the middle of gestation and active lactancy. Concerning committed cell markers, we detected a rise in AP at beginning of lactancy (d1L). We performed a BrdU uptake analysis in MZ and AP cells. The highest level of BrdU uptake was observed in MZ AT cells, whereas in AP this was detected in d1L, followed by a decrease in both the MZ and AP. Finally, we detected double immunostaining for BrdU-GFRa2 in MZ AT cells and BrdU-Sox9 in the AP d1L cells. Taken together, we hypothesize that the expansion of the SC/P niche took place mainly in MZ from pituitary rats in AT and d1L. These results suggest that the SC niche actively participates in pituitary plasticity during these reproductive states, contributing to the origin of hormone cell populations.
Palabras clave:
Pituitary Gland
,
Stems Cells
,
Marginal Zone
,
Adenoparenchyma
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Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Vaca, Alicia Maldré del Valle; Guido, Carolina Beatriz; Sosa, Liliana del Valle; Nicola, Juan Pablo; Mukdsi, Jorge Humberto; et al.; The expansion of adult stem/progenitor cells and their marker expression fluctuations are linked with pituitary plastic adaptation during gestation and lactancy; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 311; 2; 8-2016; 367-379
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