Artículo
Compartmentalization of hepatitis C virus variants in patients with hepatocellular carcinoma
Pérez, Paula Soledad
; Di Lello, Federico Alejandro
; Mullen, Eduardo; Galdame, Omar; Livellara, Beatriz I.; Gadano, Adrián Carlos; Campos, Rodolfo Hector
; Flichman, Diego Martin
Fecha de publicación:
02/2017
Editorial:
Wiley-liss, Div John Wiley & Sons Inc
Revista:
Molecular Carcinogenesis
ISSN:
0899-1987
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Chronic Hepatitis C Virus (HCV) infection is a major risk for hepatocellular carcinoma (HCC) development. HCV Core protein has been associated with the modulation of potentially oncogenic cellular processes and E2 protein has been useful in evolutive studies to analyze the diversity of HCV. Thus, the aim of this study was to evaluate HCV compartmentalization in tumoral, non-tumoral liver tissue and serum and to identify viral mutations potentially involved in carcinogenesis. Samples were obtained from four patients with HCC who underwent liver transplantation. Core and E2 were amplified, cloned and sequenced. Phylogenies and BaTS Test were performed to analyze viral compartmentalization and a signature sequence analysis was conducted by VESPA. The likelihood and Bayesian phylogenies showed a wide degree of compartmentalization in the different patients, ranging from total clustering to a more scattered pattern with small groups. Nevertheless, the association test showed compartmentalization for the three compartments and both viral regions tested in all the patients. Signature amino acid pattern supported the compartmentalization in three of the cases for E2 protein and in two of them for Core. Changes observed in Core included polymorphism R70Q/H previously associated with HCC. In conclusion, evidence of HCV compartmentalization in the liver of HCC patients was provided and further biological characterization of these variants may contribute to the understanding of carcinogenesis mediated by HCV infection.
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Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Pérez, Paula Soledad; Di Lello, Federico Alejandro; Mullen, Eduardo; Galdame, Omar; Livellara, Beatriz I.; et al.; Compartmentalization of hepatitis C virus variants in patients with hepatocellular carcinoma; Wiley-liss, Div John Wiley & Sons Inc; Molecular Carcinogenesis; 56; 2; 2-2017; 371-380
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