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dc.contributor.author
Cuellar Rodriguez, Luz Angela
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Prieto, Eduardo Daniel
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Cabaleiro, Laura Virginia
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Garda, Horacio Alberto
dc.date.available
2016-03-14T15:33:46Z
dc.date.issued
2013-11-05
dc.identifier.citation
Cuellar Rodriguez, Luz Angela; Prieto, Eduardo Daniel; Cabaleiro, Laura Virginia; Garda, Horacio Alberto; Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process; Elsevier; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1841; 1; 05-11-2013; 180-189
dc.identifier.issn
1388-1981
dc.identifier.uri
http://hdl.handle.net/11336/4766
dc.description.abstract
Discoidal high-density lipoproteins (D-HDL) are critical intermediates in reverse cholesterol transport. Most of the present knowledge of D-HDL is based on studies with reconstituted lipoprotein complexes of apolipoprotein A-I (apoA-I) obtained by cholate dialysis (CD). D-HDL can also be generated by the direct microsolubilization (DM) of phospholipid vesicles at the gel/fluid phase transition temperature, a process mechanistically similar to the "in vivo" apoAI lipidation via ABCA1. We compared the apoA-I configuration in D-HDL reconstituted with dimyristoylphosphatidylcholine by both procedures using fluorescence resonance energy transfer measurements with apoA-I tryptophan mutants and fluorescently labeled cysteine mutants. Results indicate that apoA-I configuration in D-HDL depends on the reconstitution process and are consistent with a "double belt" molecular arrangement with different helix registry. As reported by others, a configuration with juxtaposition of helices 5 of each apoAI monomer (5/5 registry) predominates in D-HDL obtained by CD. However, a configuration with helix 5 of one monomer juxtaposed with helix 2 of the other (5/2 registry) would predominate in D-HDL generated by DM. Moreover, we also show that the kinetics of cholesterol efflux from macrophage cultures depends on the reconstitution process, suggesting that apoAI configuration is important for this HDL function.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Apolipoproteins
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Fluorescence Resonance Energy Transfer (Fret)
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Lipoprotein Structure
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Site Directed Mutagenesis
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Single Tryptophan Mutants
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Cysteine Mutants
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Apolipoprotein A-I configuration and cell cholesterol efflux activity of discoidal lipoproteins depend on the reconstitution process
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
1841
dc.journal.number
1
dc.journal.pagination
180-189
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Cuellar Rodriguez, Luz Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina. Universidad Nacional de La Plata; Argentina
dc.description.fil
Fil: Prieto, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
dc.description.fil
Fil: Cabaleiro, Laura Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina. Universidad Nacional de La Plata; Argentina
dc.description.fil
Fil: Garda, Horacio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina. Universidad Nacional de La Plata; Argentina
dc.journal.title
Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbalip.2013.10.017
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info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1388198113002400
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info:eu-repo/semantics/altIdentifier/issn/1388-1981