Artículo
First genetic characterization of Fasciola hepatica in Argentina by nuclear and mitochondrial gene markers
Carnevale, Silvana
; Malandrini, Jorge Bruno; Pantano, María Laura; Soria, Claudia Cecilia; Rodrigues Silva, Rosângela; Machado Silva, José Roberto; Velásquez, Jorge Néstor; Kamenetzky, Laura
![Icon](/themes/CONICETDigital/images/conicet.png)
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Fecha de publicación:
10/2017
Editorial:
Elsevier Science
Revista:
Veterinary Parasitology
ISSN:
0304-4017
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Fasciola hepatica is a trematode showing genetic variation among isolates from different regions of the world. The objective of this work was to characterize for the first time F. hepatica isolates circulating in different regions of Argentina. Twenty-two adult flukes were collected from naturally infected bovine livers in different areas from Argentina and used for DNA extraction. We carried out PCR amplification and sequence analysis of the ribosomal internal transcribed spacer 1 (ITS1), mitochondrial nicotinamide adenine dinucleotide dehydrogenase subunits 4 and 5 (nad4 and nad5) and mitochondrial cytochrome c oxidase subunit I (cox1) genes as genetic markers. Phylogenies were reconstructed using maximum parsimony algorithm. A total of 6 haplotypes were found for cox1, 4 haplotypes for nad4 and 3 haplotypes for nad5. The sequenced ITS1 fragment was identical in all samples. The analyzed cox1 gene fragment is the most variable marker and is recommended for future analyses. No geographic association was found in the Argentinean samples.
Palabras clave:
Fasciola Hepatica
,
Phylogenetic Analysis
,
Molecular Markers
,
Its1
,
Nad4
,
Nad5
,
Cox1
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Identificadores
Colecciones
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Citación
Carnevale, Silvana; Malandrini, Jorge Bruno; Pantano, María Laura; Soria, Claudia Cecilia; Rodrigues Silva, Rosângela; et al.; First genetic characterization of Fasciola hepatica in Argentina by nuclear and mitochondrial gene markers; Elsevier Science; Veterinary Parasitology; 245; 10-2017; 34-38
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