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dc.contributor.author
Rodriguez, J.
dc.contributor.author
Soria, F.
dc.contributor.author
Geronazzo, Hugo Isidoro
dc.contributor.author
Destefanis, Hugo Alberto
dc.date.available
2016-03-11T19:53:11Z
dc.date.issued
2014-10
dc.identifier.citation
Rodriguez, J.; Soria, F.; Geronazzo, Hugo Isidoro; Destefanis, Hugo Alberto; α-Amylase Aspergillus oryzae Immobilized on Modified Expanded Perlite; De Gruyter; International Journal Of Chemical Reactor Engineering; 12; 1; 10-2014; 2194-5748
dc.identifier.issn
1542-6580
dc.identifier.uri
http://hdl.handle.net/11336/4752
dc.description.abstract
The α-amylase from Aspergillus oryzae was immobilized covalently onto expanded perlite (EP) and modified EP by treatment with TiO2 (EP-TiO2), dye HE3B (EP-HE3B) polyethylene terephthalate (PET)-hydrazide (EP-PET) and magnetite (EP-magnetite). The modified EP was characterized using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The supports were functionalized with aminopropyltriethoxysilane (APTES) and glutaraldehyde (GA). The optimum pH for free and immobilized α-amylase was 5.5. Temperature of maximum activity for free enzyme and immobilized enzyme on EP-HE3B was 50°C. The immobilized enzyme in EP-APTES this value was 55°C. The immobilized α-amylase in EP-APTES and EP-HE3B-APTES exhibited better thermostability than free enzyme. The immobilized derivatives showed moderate operational stability by retaining 50% of initial activity after seven successive reuses.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
De Gruyter
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Perlitas
dc.subject
Zeolitas
dc.subject
Enzima Inmovilizada
dc.subject
Α-Amilasa
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
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Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
α-Amylase Aspergillus oryzae Immobilized on Modified Expanded Perlite
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
12
dc.journal.number
1
dc.journal.pagination
2194-5748
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Rodriguez, J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina
dc.description.fil
Fil: Soria, F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina
dc.description.fil
Fil: Geronazzo, Hugo Isidoro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina
dc.description.fil
Fil: Destefanis, Hugo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina
dc.journal.title
International Journal Of Chemical Reactor Engineering
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.degruyter.com/view/j/ijcre.2014.12.issue-1/ijcre-2013-0145/ijcre-2013-0145.xml
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1515/ijcre-2013-0145
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/issn/1542-6580
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