Mostrar el registro sencillo del ítem

dc.contributor.author
Rodriguez, J.  
dc.contributor.author
Soria, F.  
dc.contributor.author
Geronazzo, Hugo Isidoro  
dc.contributor.author
Destefanis, Hugo Alberto  
dc.date.available
2016-03-11T19:53:11Z  
dc.date.issued
2014-10  
dc.identifier.citation
Rodriguez, J.; Soria, F.; Geronazzo, Hugo Isidoro; Destefanis, Hugo Alberto; α-Amylase Aspergillus oryzae Immobilized on Modified Expanded Perlite; De Gruyter; International Journal Of Chemical Reactor Engineering; 12; 1; 10-2014; 2194-5748  
dc.identifier.issn
1542-6580  
dc.identifier.uri
http://hdl.handle.net/11336/4752  
dc.description.abstract
The α-amylase from Aspergillus oryzae was immobilized covalently onto expanded perlite (EP) and modified EP by treatment with TiO2 (EP-TiO2), dye HE3B (EP-HE3B) polyethylene terephthalate (PET)-hydrazide (EP-PET) and magnetite (EP-magnetite). The modified EP was characterized using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The supports were functionalized with aminopropyltriethoxysilane (APTES) and glutaraldehyde (GA). The optimum pH for free and immobilized α-amylase was 5.5. Temperature of maximum activity for free enzyme and immobilized enzyme on EP-HE3B was 50°C. The immobilized enzyme in EP-APTES this value was 55°C. The immobilized α-amylase in EP-APTES and EP-HE3B-APTES exhibited better thermostability than free enzyme. The immobilized derivatives showed moderate operational stability by retaining 50% of initial activity after seven successive reuses.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
De Gruyter  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Perlitas  
dc.subject
Zeolitas  
dc.subject
Enzima Inmovilizada  
dc.subject
Α-Amilasa  
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación  
dc.subject.classification
Biotecnología Industrial  
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS  
dc.title
α-Amylase Aspergillus oryzae Immobilized on Modified Expanded Perlite  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2016-03-30 10:35:44.97925-03  
dc.journal.volume
12  
dc.journal.number
1  
dc.journal.pagination
2194-5748  
dc.journal.pais
Alemania  
dc.journal.ciudad
Berlin  
dc.description.fil
Fil: Rodriguez, J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina  
dc.description.fil
Fil: Soria, F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina  
dc.description.fil
Fil: Geronazzo, Hugo Isidoro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina  
dc.description.fil
Fil: Destefanis, Hugo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina  
dc.journal.title
International Journal Of Chemical Reactor Engineering  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.degruyter.com/view/j/ijcre.2014.12.issue-1/ijcre-2013-0145/ijcre-2013-0145.xml  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1515/ijcre-2013-0145  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/issn/1542-6580