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Artículo

Integrated process for the purification and immobilization of the envelope protein domain III of dengue virus type 2 expressed in Rachiplusia nu larvae and its potential application in a diagnostic assay

Smith, María EmiliaIcon ; Targovnik, Alexandra MarisaIcon ; Cerezo, JulietaIcon ; Morales, Maria AlejandraIcon ; Miranda, Maria VictoriaIcon ; Rodriguez Talou, JulianIcon
Fecha de publicación: 03/2017
Editorial: Academic Press Inc Elsevier Science
Revista: Protein Expression and Purification
ISSN: 1046-5928
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biotecnología Industrial

Resumen

Dengue incidence has grown dramatically in the last years, with about 40% of the world population at risk of infection. Recently, a vaccine developed by Sanofi Pasteur has been registered, but only in a few countries. Moreover, specific antiviral drugs are not available. Thus, an efficient and accurate diagnosis is important for disease management. To develop a low-cost immunoassay for dengue diagnosis, in the present study we expressed the envelope protein domain III of dengue virus type 2 in Rachiplusia nu larvae by infection with a recombinant baculovirus. The antigen was expressed as a fusion to hydrophobin I (DomIIIHFBI) to easily purify it by an aqueous two-phase system (ATPS) and to efficiently immobilize it in immunoassay plates. A high level of recombinant DomIIIHFBI was obtained in R. nu, where yields reached 4.5 mg per g of larva. Also, we were able to purify DomIIIHFBI by an ATPS with 2% of Triton X-114, reaching a yield of 73% and purity higher than 80% in a single purification step. The recombinant DomIIIHFBI was efficiently immobilized in hydrophobic surface plates. The immunoassay we developed with the immobilized antigen was able to detect IgG specific for dengue virus type 2 in serum samples and not for other serotypes.
Palabras clave: Dengue Virus Antigen , Insect Larvae , Recombinant Fusion Protein; , Hydrophobins , Diagnostic Immunoassay
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/47493
DOI: https://dx.doi.org/10.1016/j.pep.2016.11.007
URL: https://www.sciencedirect.com/science/article/pii/S1046592816304090
Colecciones
Articulos(NANOBIOTEC)
Articulos de INSTITUTO DE NANOBIOTECNOLOGIA
Citación
Smith, María Emilia; Targovnik, Alexandra Marisa; Cerezo, Julieta; Morales, Maria Alejandra; Miranda, Maria Victoria; et al.; Integrated process for the purification and immobilization of the envelope protein domain III of dengue virus type 2 expressed in Rachiplusia nu larvae and its potential application in a diagnostic assay; Academic Press Inc Elsevier Science; Protein Expression and Purification; 131; 3-2017; 76-84
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