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Artículo

Implementation of new tools in molecular epidemiology studies of Echinococcus granulosus sensu lato in South America

Avila, Héctor GabrielIcon ; Santos, Guilherme B.; Cucher, Marcela AlejandraIcon ; Macchiaroli, NataliaIcon ; Pérez, Matías GastónIcon ; Baldi, GermánIcon ; Jensen, Oscar; Pérez, Matías GastónIcon ; López, Raúl; Negro, Perla Susana; Scialfa, Exequiel Alejandro; Zaha, Arnaldo; Ferreira, Henrique B.; Rosenzvit, Mara CeciliaIcon ; Kamenetzky, LauraIcon
Fecha de publicación: 06/2017
Editorial: Elsevier Ireland
Revista: Parasitology International
ISSN: 1383-5769
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Salud Ocupacional

Resumen

The aim of this work was to determine Echinococcus granulosus sensu lato species and genotypes in intermediate and definitive hosts and in human isolates from endemic regions of Argentina and Brazil including those where no molecular data is available by a combination of classical and alternative molecular tools. A total of 227 samples were isolated from humans, natural intermediate and definitive hosts. Amplification of cytochrome c oxidase subunit I gene fragment was performed and a combination of AluI digestion assay, High Resolution Melting anal- ysis (HRM) assay and DNA sequencing was implemented for Echinococcus species/genotype determination. E. granulosus sensu stricto (G1) was found in sheep (n = 35), cattle (n = 67) and dogs (n = 5); E. ortleppi (G5) in humans (n = 3) and cattle (n = 108); E. canadensis (G6) in humans (n = 2) and E. canadensis (G7) in pigs (n = 7). We reported for the first time the presence of E. ortleppi (G5) and E. canadensis (G6) in humans from San Juan and Catamarca Argentinean provinces and E. canadensis (G7) in pigs from Cordoba Argentinean province. In this work, we widened molecular epidemiology studies of E. granulosus s. l. in South America by an- alyzing several isolates from definitive and intermediate hosts, including humans from endemic regions were such information was scarce or unavailable. The presence of different species/genotypes in the same region and host species reinforce the need of rapid and specific techniques for accurate determination of Echinococcus species such as the ones proposed in this work.
Palabras clave: Echinococcus Granulosus Sensu Lato , South America , Genotypes , Neglected Disease , Echinococcosis
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/47473
DOI: https://dx.doi.org/10.1016/j.parint.2017.02.001
URL: https://www.sciencedirect.com/science/article/pii/S138357691630438X
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Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Citación
Avila, Héctor Gabriel; Santos, Guilherme B.; Cucher, Marcela Alejandra; Macchiaroli, Natalia; Pérez, Matías Gastón; et al.; Implementation of new tools in molecular epidemiology studies of Echinococcus granulosus sensu lato in South America; Elsevier Ireland; Parasitology International; 66; 3; 6-2017; 250-257
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