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dc.contributor.author
Rey Funes, Manuel  
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Larráyoz, Ignacio M.  
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Fernández, Juan C.  
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Contartese, Daniela Soledad  
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Rolón, Federico  
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Inserra, Pablo Ignacio Felipe  
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Martínez Murillo, Ricardo  
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López, Juan José  
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Dorfman, Verónica Berta  
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Martínez, Alfredo  
dc.contributor.author
Loidl, Cesar Fabian  
dc.date.available
2018-06-05T20:47:51Z  
dc.date.issued
2016-06  
dc.identifier.citation
Rey Funes, Manuel; Larráyoz, Ignacio M.; Fernández, Juan C.; Contartese, Daniela Soledad; Rolón, Federico; et al.; Methylene blue prevents retinal damage in an experimental model of ischemic proliferative retinopathy; American Physiological Society; American Journal Of Physiology-regulatory, Integrative And Comparative Physiology; 310; 11; 6-2016; 1011-1019  
dc.identifier.issn
0363-6119  
dc.identifier.uri
http://hdl.handle.net/11336/47408  
dc.description.abstract
Perinatal asphyxia induces retinal lesions, generating ischemic proliferative retinopathy, which may result in blindness. Previously, we showed that the nitrergic system was involved in the physiopathology of perinatal asphyxia. Here we analyze the application of methylene blue, a well-known soluble guanylate cyclase inhibitor, as a therapeutic strategy to prevent retinopathy. Male rats (n = 28 per group) were treated in different ways: 1) control group comprised born-to-term animals; 2) methylene blue group comprised animals born from pregnant rats treated with methylene blue (2 mg/kg) 30 and 5 min before delivery; 3) perinatal asphyxia (PA) group comprised rats exposed to perinatal asphyxia (20 min at 37°C); and 4) methylene blue-PA group comprised animals born from pregnant rats treated with methylene blue (2 mg/kg) 30 and 5 min before delivery, and then the pups were subjected to PA as above. For molecular studies, mRNA was obtained at different times after asphyxia, and tissue was collected at 30 days for morphological and biochemical analysis. Perinatal asphyxia produced significant gliosis, angiogenesis, and thickening of the inner retina. Methylene blue treatment reduced these parameters. Perinatal asphyxia resulted in a significant elevation of the nitrergic system as shown by NO synthase (NOS) activity assays, Western blotting, and (immuno)histochemistry for the neuronal isoform of NOS and NADPH-diaphorase activity. All these parameters were also normalized by the treatment. In addition, methylene blue induced the upregulation of the anti-angiogenic peptide, pigment epithelium-derived factor. Application of methylene blue reduced morphological and biochemical parameters of retinopathy. This finding suggests the use of methylene blue as a new treatment to prevent or decrease retinal damage in the context of ischemic proliferative retinopathy.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
American Physiological Society  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Angiogenesis  
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Ischemic Proliferative Retinopathy  
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Methylene Blue  
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Nitric Oxide  
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Retina  
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Neurociencias  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Methylene blue prevents retinal damage in an experimental model of ischemic proliferative retinopathy  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-05-28T14:50:35Z  
dc.journal.volume
310  
dc.journal.number
11  
dc.journal.pagination
1011-1019  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Bethesda  
dc.description.fil
Fil: Rey Funes, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina  
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Fil: Larráyoz, Ignacio M.. Angiogenesis Study Group. Center for Biomedical Research of La Rioja; España  
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Fil: Fernández, Juan C.. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina  
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Fil: Contartese, Daniela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina  
dc.description.fil
Fil: Rolón, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina  
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Fil: Inserra, Pablo Ignacio Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico. Departamento de Estudios Biomédicos y Biotecnológicos; Argentina  
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Fil: Martínez Murillo, Ricardo. Consejo Superior de Investigaciones Científicas; España  
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Fil: López, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina  
dc.description.fil
Fil: Dorfman, Verónica Berta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico. Departamento de Estudios Biomédicos y Biotecnológicos; Argentina  
dc.description.fil
Fil: Martínez, Alfredo. Angiogenesis Study Group. Center for Biomedical Research of La Rioja; España  
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Fil: Loidl, Cesar Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina. Universidad Católica de Cuyo - Sede San Juan. Facultad de Ciencias Médicas. Departamento de Neurociencia; Argentina  
dc.journal.title
American Journal Of Physiology-regulatory, Integrative And Comparative Physiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1152/ajpregu.00266.2015  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.physiology.org/doi/10.1152/ajpregu.00266.2015