Artículo
Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography
Fecha de publicación:
01/2017
Editorial:
Elsevier Science
Revista:
Microchemical Journal
ISSN:
0026-265X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In this work, the interaction between synthetic bioactive peptides and polymeric nanoparticles (NPs) used as pseudostationary phase in capillary electrochromatography was studied. NPs were prepared from methacrylic acid and ethylenglycol dimethacrylate with benzoyl peroxide by utilizing a precipitation polymerization technique. The reaction was monitored by infrared spectroscopy. Polymer characterization was performed by dynamic light scattering and transmission electron microscopy. Experimental running conditions were tested, including organic solvent proportion in the background electrolyte, capillary conditioning, applied voltage, sample introduction amount, and how NPs were incorporated into the system. A continuous full filling technique in which the NPs were suspended in the entire electrolyte volume as well as a conventional partial filling technique were used. Results obtained at pH 7.0 suggest that the NPs have a very strong interaction with more basic peptides. The interaction between analytes and NPs was found to be predominantly ionic.
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Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Grela, Denise Agata; Zannoni, Valeria; Vizioli, Nora Matilde; Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography; Elsevier Science; Microchemical Journal; 130; 1-2017; 153-156
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