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Artículo

Systemic Transplantation of Bone Marrow Mononuclear Cells Promotes Axonal Regeneration and Analgesia in a Model of Wallerian Degeneration

Usach, VaninaIcon ; Malet, MarianaIcon ; Lopez, Lidia MargaritaIcon ; Lavalle, Lucía; Piñero, Gonzalo MiguelIcon ; Saccoliti, María; Cueto, Alicia; Brumovsky, Pablo RodolfoIcon ; Brusco, Herminia AliciaIcon ; Setton, Clara PatriciaIcon
Fecha de publicación: 12/2016
Editorial: Lippincott Williams
Revista: Transplantation
ISSN: 0041-1337
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Medicina Básica

Resumen

BACKGROUND: Reinnervation timing after nerve injury is critical for favorable axonal regeneration, remyelination and clinical improvement. Considering bone marrow mononuclear cells (BMMC) are easily obtained and readily available for transplant, this work analyzed the effect of BMMC systemic administration on nerve repair and pain behavior.METHODS: Adult rats with sciatic nerve crush were immediately and systemically injected BMMC through the caudal artery. Nontreated, sham and naïve rats were also included. Histological, immunohistochemical, biochemical, functional and behavioral analyses were performed in nerves harvested from each group at different survival times.RESULTS: Axons in BMMC-treated rats exhibited a more conserved morphological appearance than those in nontreated rats, as observed at different survival times both in semi-thin sections and ultrastructural analysis. BMMC-treated rats also showed a reduction in major myelin protein immunoreactive clusters 7 and 14 days post injury (DPI), as compared to nontreated rats. Electrophysiological analysis showed BMMC treatment to slightly improve the amplitude of compound muscle action potential (CMAP) starting at 14 DPI. Finally, mechanical withdrawal threshold revealed a full preventive action against transient mechanical hypersensitivity in BMMC-treated rats.CONCLUSIONS: These data demonstrate the efficiency of BMMC, systemically and noninvasively transplanted, in correcting morphological, functional and behavioral alterations resulting from peripheral nerve injury.
Palabras clave: Mononuclear Cells , Systemic Transplantation , Axonal Regeneration , Wallerian Degeneration , Systemic Transplantation , Axonal Regeneration , Schwann Cell , Bone Marrow
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/47221
DOI: http://dx.doi.org/10.1097/TP.0000000000001478
URL: https://insights.ovid.com/pubmed?pmid=27607534
URL: https://journals.lww.com/transplantjournal/Abstract/2017/07000/Systemic_Transpla
Colecciones
Articulos(IBCN)
Articulos de INST.DE BIOLO.CEL.Y NEURCS."PROF.E.DE ROBERTIS"
Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Usach, Vanina; Malet, Mariana; Lopez, Lidia Margarita; Lavalle, Lucía; Piñero, Gonzalo Miguel; et al.; Systemic Transplantation of Bone Marrow Mononuclear Cells Promotes Axonal Regeneration and Analgesia in a Model of Wallerian Degeneration; Lippincott Williams; Transplantation; 101; 7; 12-2016; 1573-1586
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