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dc.contributor.author
Reischer, Georg H.
dc.contributor.author
Ebdon, James E.
dc.contributor.author
Bauer, Johanna M.
dc.contributor.author
Schuster, Nathalie
dc.contributor.author
Ahmed, Warish
dc.contributor.author
Åström, Johan
dc.contributor.author
Blanch, Anicet R.
dc.contributor.author
Blöschl, Günter
dc.contributor.author
Byamukama, Denis
dc.contributor.author
Coakley, Tricia
dc.contributor.author
Ferguson, Christobel
dc.contributor.author
Goshu, Goraw
dc.contributor.author
Ko, GwangPyo
dc.contributor.author
de Roda Husman, Ana Maria
dc.contributor.author
Mushi, Douglas
dc.contributor.author
Poma, Hugo Ramiro
dc.contributor.author
Pradhan, Bandana
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Rajal, Verónica Beatriz
dc.contributor.author
Schade, Margit A.
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Sommer, Regina
dc.contributor.author
Taylor, Huw
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Toth, Erika M.
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Vrajmasu, Virgil
dc.contributor.author
Wuertz, Stefan
dc.contributor.author
Mach, Robert L.
dc.contributor.author
Farnleitner, Andreas H.
dc.date.available
2016-03-10T20:06:02Z
dc.date.issued
2013-06
dc.identifier.citation
Reischer, Georg H.; Ebdon, James E.; Bauer, Johanna M.; Schuster, Nathalie; Ahmed, Warish; et al.; Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents; American Chemical Society; Environmental Science & Technology; 47; 15; 6-2013; 8548-8556
dc.identifier.issn
0013-936X
dc.identifier.uri
http://hdl.handle.net/11336/4720
dc.description.abstract
Numerous quantitative PCR assays for microbial fecal source tracking (MST) have been developed and evaluated in recent years. Widespread application has been hindered by a lack of knowledge regarding the geographical stability and hence applicability of such methods beyond the regional level. This study assessed the performance of five previously reported quantitative PCR assays targeting human-, cattle- or ruminant-associated Bacteroidetes populations on 280 human and animal fecal samples from 16 countries across six continents. The tested cattle-associated markers were shown to be ruminant-associated. The quantitative distributions of marker concentrations in target and non target samples proved to be essential for the assessment of assay performance and were used to establish a new metric for quantitative source-specificity. In general, this study demonstrates that stable target populations required for marker-based MST occur around the globe. Ruminant-associated marker concentrations were strongly correlated with total intestinal Bacteroidetes populations and with each other, indicating that the detected ruminant-associated populations seem to be part of the intestinal core microbiome of ruminants worldwide. Consequently tested ruminant targeted assays appear to be suitable quantitative MST tools beyond the regional level while the targeted human-associated populations seem to be less prevalent and stable suggesting potential for improvements in human-targeted methods.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Microbial Source Tracking
dc.subject
Pollution Microbiology
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Fecal Pollution
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Bacteroidetes
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Quantitative Real-Time Pcr
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Genetic Markers
dc.subject
Water Quality
dc.subject.classification
Biotecnología Medioambiental
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Biotecnología del Medio Ambiente
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Performance assessment of qPCR assays targeting human- and ruminant-associated Bacteroidetes for microbial source tracking across sixteen countries on six continents
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
47
dc.journal.number
15
dc.journal.pagination
8548-8556
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Reischer, Georg H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; Austria
dc.description.fil
Fil: Ebdon, James E.. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino Unido
dc.description.fil
Fil: Bauer, Johanna M.. Vienna University of Technology; Austria
dc.description.fil
Fil: Schuster, Nathalie. Vienna University of Technology; Austria
dc.description.fil
Fil: Ahmed, Warish. CSIRO Land and Water; Australia
dc.description.fil
Fil: Åström, Johan. Chalmers University Of Technology; Suecia
dc.description.fil
Fil: Blanch, Anicet R.. Universidad de Barcelona. Departamento de Bioquimica; España
dc.description.fil
Fil: Blöschl, Günter. Vienna University of Technology; Austria
dc.description.fil
Fil: Byamukama, Denis. Makerere University. Department of Biochemistry; Uganda
dc.description.fil
Fil: Coakley, Tricia. University Of Kentucky; Estados Unidos
dc.description.fil
Fil: Ferguson, Christobel. ALS Water Sciences Group; Australia
dc.description.fil
Fil: Goshu, Goraw. Bahir Dar University. Blue Nile Water Institute. College of Agriculture and Environmental Sciences; Etiopía
dc.description.fil
Fil: Ko, GwangPyo. Seoul National University; Corea del Sur
dc.description.fil
Fil: de Roda Husman, Ana Maria. National Institute for Public Health and the Environment ; Países Bajos
dc.description.fil
Fil: Mushi, Douglas. Sokoine University; Tanzania
dc.description.fil
Fil: Poma, Hugo Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina
dc.description.fil
Fil: Pradhan, Bandana. Tribhuvan University; Nepal
dc.description.fil
Fil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina
dc.description.fil
Fil: Schade, Margit A.. Bavarian Environment Agency; Alemania
dc.description.fil
Fil: Sommer, Regina. InterUniversity Cooperation Centre Water & Health; Austria. Medical University of Vienna. Institute for Hygiene and Applied Immunology, Water Hygiene; Austria
dc.description.fil
Fil: Taylor, Huw. University of Brighton. School of Environment and Technology. Environment & Public Health Research Unit ; Reino Unido
dc.description.fil
Fil: Toth, Erika M.. Eötvös Lorand University. Department of Microbiology. Biological Institute; Hungría
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Fil: Vrajmasu, Virgil. Veterinary State Laboratory; Rumania
dc.description.fil
Fil: Wuertz, Stefan. University Of California At Davis; Estados Unidos
dc.description.fil
Fil: Mach, Robert L.. Vienna University of Technology; Austria
dc.description.fil
Fil: Farnleitner, Andreas H.. Vienna University of Technology; Austria. InterUniversity Cooperation Centre Water & Health; Austria
dc.journal.title
Environmental Science & Technology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/es304367t
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021%2Fes304367t
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