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dc.contributor.author Bustamante, Ana Victoria
dc.contributor.author Sanso, Andrea Mariel
dc.contributor.author Segura, D. O.
dc.contributor.author Parma, Alberto Ernesto
dc.contributor.author Lucchesi, Paula Maria Alejandra
dc.date.available 2016-03-09T17:57:02Z
dc.date.issued 2013-07
dc.identifier.citation Bustamante, Ana Victoria; Sanso, Andrea Mariel; Segura, D. O.; Parma, Alberto Ernesto; Lucchesi, Paula Maria Alejandra; Dynamic of Mutational Events in Variable Number Tandem Repeats of Escherichia coli O157:H7; Hindawi Publishing Corporation; BioMed Research International; 2013; 7-2013; 390354-390354
dc.identifier.issn 2314-6141
dc.identifier.uri http://hdl.handle.net/11336/4706
dc.description.abstract VNTRs regions have been successfully used for bacterial subtyping; however, the hypervariability in VNTR loci is problematic when trying to predict the relationships among isolates. Since few studies have examined the mutation rate of these markers, our aim was to estimate mutation rates of VNTRs specific for verotoxigenic E. coli O157:H7. The knowledge of VNTR mutational rates and the factors affecting them would make MLVA more effective for epidemiological or microbial forensic investigations. For this purpose, we analyzed nine loci performing parallel, serial passage experiments (PSPEs) on 9 O157:H7 strains. The combined 9 PSPE population rates for the 8 mutating loci ranged from 4.4 × 10−05 to 1.8 × 10−03 mutations/generation, and the combined 8-loci mutation rate was of 2.5 × 10−03 mutations/generation. Mutations involved complete repeat units, with only one point mutation detected. A similar proportion between single and multiple repeat changes was detected. Of the 56 repeat mutations, 59% were insertions and 41% were deletions, and 72% of the mutation events corresponded to O157-10 locus. For alleles with up to 13 UR, a constant and low mutation rate was observed; meanwhile longer alleles were associated with higher and variable mutation rates. Our results are useful to interpret data from microevolution and population epidemiology studies and particularly point out that the inclusion or not of O157-10 locus or, alternatively, a differential weighting data according to the mutation rates of loci must be evaluated in relation with the objectives of the proposed study.
dc.format application/pdf
dc.language.iso eng
dc.publisher Hindawi Publishing Corporation
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject VNTRs
dc.subject Escherichia coli O157:H7
dc.subject Mutation rate
dc.subject Bacterial subtyping
dc.subject.classification Ciencias Veterinarias
dc.subject.classification Ciencias Veterinarias
dc.subject.classification CIENCIAS AGRÍCOLAS
dc.title Dynamic of Mutational Events in Variable Number Tandem Repeats of Escherichia coli O157:H7
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2016-03-30 10:35:44.97925-03
dc.journal.volume 2013
dc.journal.pagination 390354-390354
dc.journal.pais Estados Unidos
dc.journal.ciudad New York
dc.description.fil Fil: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
dc.description.fil Fil: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
dc.description.fil Fil: Segura, D. O.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
dc.description.fil Fil: Parma, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
dc.description.fil Fil: Lucchesi, Paula Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
dc.journal.title BioMed Research International
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/bmri/2013/390354/
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1155/2013/390354
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/issn/2314-6141


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)