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dc.contributor.author Mamone, Leandro Ariel
dc.contributor.author Di Venosa, Gabriela Mariana
dc.contributor.author Saenz, Daniel Alberto
dc.contributor.author Batlle, Alcira María del C.
dc.contributor.author Casas, Adriana Gabriela
dc.date.available 2018-06-01T19:51:06Z
dc.date.issued 2016-10
dc.identifier.citation Mamone, Leandro Ariel; Di Venosa, Gabriela Mariana; Saenz, Daniel Alberto; Batlle, Alcira María del C.; Casas, Adriana Gabriela; Methods for the detection of reactive oxygen species employed in the identification of plant photosensitizers; Academic Press Inc Elsevier Science; Methods; 109; 10-2016; 73-80
dc.identifier.issn 1046-2023
dc.identifier.uri http://hdl.handle.net/11336/47027
dc.description.abstract Over the past ten years, alternative methods for the rapid screening of PSs have been developed. In the present work, a study was undertaken to correlate the phototoxicity of plant extracts on either prokaryotic or eukaryotic cells, with the total oxidation status (TOS) as well as with their ability to produce 1O2. Results demonstrated that the extracts containing PSs that were active either on eukaryotic cells or bacteria increased their TOS after illumination, and that there was a certain degree of positive correlation between the extract phototoxic efficacy and TOS levels. The production of 1O2 by the illuminated extracts was indirectly measured by the use of the fluorescence of “singlet oxygen sensor green”, which is a method that has proved highly sensitive for such measurement. 1O2 was detectable only upon illumination of the most active extracts. In addition, the oxidation of tryptophan and was employed as a method capable of measuring ROS generated by both type I and II ROS reactions. However, it turned out to be not sensitive enough to detect the species generated by plant extracts. Results demonstrated that the TOS method, initially developed to measure the oxidant status in plasma, can be readily applied to plant extracts. Unlike the method used to detect 1O2, the method employed for the detection of TOS proved to be accurate, since all the extracts that displayed a high phototoxic activity on either prokaryotic or eukaryotic cells, presented high TOS levels after illumination.
dc.format application/pdf
dc.language.iso eng
dc.publisher Academic Press Inc Elsevier Science
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject FREE RADICALS
dc.subject PHOTODYNAMIC THERAPY
dc.subject PHOTODYNAMIC ANTIMICROBIAL CHEMOTHERAPY
dc.subject PLANTS
dc.subject REACTIVE OXYGEN SPECIES
dc.subject SINGLET OXYGEN
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Methods for the detection of reactive oxygen species employed in the identification of plant photosensitizers
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-06-01T19:20:39Z
dc.journal.volume 109
dc.journal.pagination 73-80
dc.journal.pais Estados Unidos
dc.journal.ciudad San Diego
dc.description.fil Fil: Mamone, Leandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
dc.description.fil Fil: Di Venosa, Gabriela Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
dc.description.fil Fil: Saenz, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
dc.description.fil Fil: Batlle, Alcira María del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
dc.description.fil Fil: Casas, Adriana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
dc.journal.title Methods
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.ymeth.2016.05.020
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1046202316301530
dc.conicet.fuente Elsevier


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    Articulos de CENTRO DE INVEST. SOBRE PORFIRINAS Y PORFIRIAS

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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)