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dc.contributor.author
Ramírez Tapias, Yuly Andrea  
dc.contributor.author
Rivero, Cintia Wanda  
dc.contributor.author
Gallego, Fernando López  
dc.contributor.author
Guisán, José M.  
dc.contributor.author
Trelles, Jorge Abel  
dc.date.available
2018-06-01T18:25:10Z  
dc.date.issued
2016-03  
dc.identifier.citation
Ramírez Tapias, Yuly Andrea; Rivero, Cintia Wanda; Gallego, Fernando López; Guisán, José M.; Trelles, Jorge Abel; Stabilization by multipoint covalent attachment of a biocatalyst with polygalacturonase activity used for juice clarification; Elsevier; Food Chemistry; 208; 3-2016; 252-257  
dc.identifier.issn
0308-8146  
dc.identifier.uri
http://hdl.handle.net/11336/47000  
dc.description.abstract
Derivatized-agarose supports are suitable for enzyme immobilization by different methods, taking advantage of different physical, chemical and biological conditions of the protein and the support. In this study, agarose particles were modified with MANAE, PEI and glyoxyl groups and evaluated to stabilize polygalacturonase from Streptomyces halstedii ATCC 10897. A new immobilized biocatalyst was developed using glyoxyl-agarose as support; it exhibited high performance in degrading polygalacturonic acid and releasing oligogalacturonides. Maximal enzyme activity was detected at 5 h of reaction using 0.05 g/mL of immobilized biocatalyst, which released 3 mg/mL of reducing sugars and allowed the highest product yield conversion and increased stability. These results are very favorable for pectin degradation with reusability up to 18 successive reactions (90 h) and application in juice clarification. Plum (4.7 °Bx) and grape (10.6 °Bx) juices were successfully clarified, increasing reducing sugars content and markedly decreasing turbidity and viscosity.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Glyoxyl-Agarose  
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Streptomyces Halstedii Atcc 10897  
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Enzyme Purification  
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Grape And Plum Juices  
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Otras Biotecnología Agropecuaria  
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Biotecnología Agropecuaria  
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CIENCIAS AGRÍCOLAS  
dc.title
Stabilization by multipoint covalent attachment of a biocatalyst with polygalacturonase activity used for juice clarification  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-05-31T15:07:30Z  
dc.journal.volume
208  
dc.journal.pagination
252-257  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Ramírez Tapias, Yuly Andrea. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina  
dc.description.fil
Fil: Rivero, Cintia Wanda. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina  
dc.description.fil
Fil: Gallego, Fernando López. Heterogeneous Biocatalysis Group; España. Ikerbasque; España  
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Fil: Guisán, José M.. Institute of Catalysis and Petrochemistry; España  
dc.description.fil
Fil: Trelles, Jorge Abel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina  
dc.journal.title
Food Chemistry  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.foodchem.2016.03.086  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814616304605