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dc.contributor.author
Costa, Sofia
dc.contributor.author
Sastre, Patricia
dc.contributor.author
Pérez, Teresa
dc.contributor.author
Tapia, Istar
dc.contributor.author
Barrandeguy, Maria Eugenia
dc.contributor.author
Sánchez-Vizcaíno, José M.
dc.contributor.author
Sánchez-Matamoros, Almudena
dc.contributor.author
Wigdorovitz, Andrés
dc.contributor.author
Sanz, Antonio
dc.contributor.author
Rueda, Paloma
dc.date.available
2018-06-01T18:24:59Z
dc.date.issued
2016-11
dc.identifier.citation
Costa, Sofia; Sastre, Patricia; Pérez, Teresa; Tapia, Istar; Barrandeguy, Maria Eugenia; et al.; Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African Horse Sickness and Equine Infectious Anemia viruses; Elsevier Science; Journal of Virological Methods; 237; 11-2016; 127-131
dc.identifier.issn
0166-0934
dc.identifier.uri
http://hdl.handle.net/11336/46998
dc.description.abstract
African horse sickness (AHS) and equine infectious anemia (EIA) are both notifiable equid specific diseases that may present similar clinical signs. Considering the increased global movement of horses and equine products over the past decades, together with the socio-economic impact of previous AHS and EIA outbreaks, there is a clear demand for an early discrimination and a strict control of their transmission between enzootic and AHS/EIA-free regions. Currently, the individual control and prevention of AHS or EIA relies on a series of measures, including the restriction of animal movements, vector control, and the use of several laboratory techniques for viral identification, amongst others. Despite being widely employed in surveillance programmes and in the control of animal movements, the available serological assays can only detect AHS- or EIA-specific antibodies individually. In this work, a duplex lateral flow assay (LFA) for simultaneous detection and differentiation of specific antibodies against AHS virus (AHSV) and EIA virus (EIAV) was developed and evaluated with experimental and field serum samples. The duplex LFA was based on the AHSV-VP7 outer core protein and the EIAV-P26 major core protein. The results indicated that the duplex LFA presented a good analytical performance, detecting simultaneously and specifically antibodies against AHSV and EIAV. The initial diagnostic evaluation revealed a good agreement with results from the AHS and EIA tests prescribed by the OIE, and it highlighted the usefulness of the new AHSV/EIAV duplex LFA for an on-field and point-of-care first diagnosis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
African Horse Sickness
dc.subject
Equine Infectious Anaemia
dc.subject
Lateral Flow Assay
dc.subject
Antibodies
dc.subject.classification
Otras Biotecnologías de la Salud
dc.subject.classification
Biotecnología de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African Horse Sickness and Equine Infectious Anemia viruses
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-05-31T15:03:17Z
dc.journal.volume
237
dc.journal.pagination
127-131
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Costa, Sofia. Inmunología y Genética Aplicada; España
dc.description.fil
Fil: Sastre, Patricia. Inmunología y Genética Aplicada; España
dc.description.fil
Fil: Pérez, Teresa. Inmunología y Genética Aplicada; España
dc.description.fil
Fil: Tapia, Istar. Inmunología y Genética Aplicada; España
dc.description.fil
Fil: Barrandeguy, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Universidad del Salvador; Argentina
dc.description.fil
Fil: Sánchez-Vizcaíno, José M.. VISAVET Health Surveillance Centre; España. Animal Health Department; España
dc.description.fil
Fil: Sánchez-Matamoros, Almudena. VISAVET Health Surveillance Centre; España. Animal Health Department; España
dc.description.fil
Fil: Wigdorovitz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
dc.description.fil
Fil: Sanz, Antonio. Inmunología y Genética Aplicada; España
dc.description.fil
Fil: Rueda, Paloma. Inmunología y Genética Aplicada; España
dc.journal.title
Journal of Virological Methods
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.jviromet.2016.09.009
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166093416301641
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