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Artículo

Biotransformation of halogenated nucleosides by immobilized Lactobacillus animalis 2′- N -deoxyribosyltransferase

Britos, Claudia NoeliaIcon ; Lapponi, María JoséIcon ; Cappa, Valeria AlejandraIcon ; Rivero, Cintia WandaIcon ; Trelles, Jorge AbelIcon
Fecha de publicación: 06/2016
Editorial: Elsevier Science Sa
Revista: Journal of Fluorine Chemistry
ISSN: 0022-1139
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Ingeniería Química

Resumen

An immobilized biocatalyst with 2′-N-deoxyribosyltransferase (NDT) activity, Lactobacillus animalis NDT (LaNDT), was developed from cell free extracts. LaNDT was purified, characterized and then immobilized by ionic interaction. Different process parameters were optimized, resulting in an active derivative (2.6 U/g) able to obtain 1.75 mg/g of 5-fluorouracil-2′-deoxyriboside, an antimetabolite known as floxuridine, used in gastrointestinal cancer treatment. Furthermore, immobilized LaNDT was satisfactorily used to obtain at short reaction times other halogenated pyrimidine and purine 2′-deoxynucleosides such as 6-chloropurine-2′-deoxyriboside (4.9 U/g), 6-bromopurine-2′-deoxyriboside (4.3 U/g), 6-chloro-2-fluoropurine-2′-deoxyriboside (5.4 U/g), 5-bromo-2′-deoxyuridine (2.8 U/g) and 5-chloro-2′-deoxyuridine (1.8 U/g) compounds of pharmaceutical interest in antiviral or antitumor treatments. Besides, increasing the biocatalyst amount 8 times per volume unit allowed obtaining a 5-fold improvement in floxuridine biotransformation. The developed biocatalyst proved to be effective for the biosynthesis of a wide spectrum of nucleoside analogues by employing an economical, simple and environmentally friendly methodology.
Palabras clave: Green Chemistry , Floxuridine , Deae-Sepharose , Antiviral Agents , Antitumor Compounds , Enzyme Stabilization
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/46978
DOI: https://dx.doi.org/10.1016/j.jfluchem.2016.04.012
URL: https://www.sciencedirect.com/science/article/pii/S0022113916301002
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Britos, Claudia Noelia; Lapponi, María José; Cappa, Valeria Alejandra; Rivero, Cintia Wanda; Trelles, Jorge Abel; Biotransformation of halogenated nucleosides by immobilized Lactobacillus animalis 2′- N -deoxyribosyltransferase; Elsevier Science Sa; Journal of Fluorine Chemistry; 186; 6-2016; 91-96
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