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Artículo

Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development

Coluccio Leskow, CarlaIcon ; Kamenetzky, LauraIcon ; Dominguez, Pia GuadalupeIcon ; Diaz Zirpolo, Jose AntonioIcon ; Obata, Toshihiro; Costa, HernánIcon ; Marti, Marcelo AdrianIcon ; Taboga, Oscar AlbertoIcon ; Keurentjes, Joost; Sulpice, Ronan; Ishihara, Hirofumi; Stitt, Mark; Fernie, Alisdair Robert; Carrari, Fernando OscarIcon
Fecha de publicación: 07/2016
Editorial: Oxford University Press
Revista: Journal of Experimental Botany
ISSN: 0022-0957
e-ISSN: 1460-2431
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Biológicas

Resumen

Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar Km for both alleles and a slightly higher Vmax for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vacInv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.
Palabras clave: Genomica , Metabolomica , Arabidopsis , Biomass , Inhibitor , Near Isogenic Line , Quantitative Trait Loci , Vacuolar Invertase
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/46969
URL: https://academic.oup.com/jxb/article/67/14/4091/2197644
DOI: https://doi.org/10.1093/jxb/erw185
Colecciones
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Coluccio Leskow, Carla; Kamenetzky, Laura; Dominguez, Pia Guadalupe; Diaz Zirpolo, Jose Antonio; Obata, Toshihiro; et al.; Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development; Oxford University Press; Journal of Experimental Botany; 67; 14; 7-2016; 4091-4103
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